Combined archive search across FBI Vault, SPLC, PHMPT, ACIP, NIH grants, the Federal Writers' Project, and other collections we've ingested.
Aleksandra Nita-Lazar · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2021 · $2,762 — The focus of the proteomic approach is to identify and quantify viral structural proteins, specifically spike (S) glycoprotein as well as envelope (E) protein, membrane (M) protein, and nucleocapsid (N) protein in patient samples. The S protein mediates attachment of the virus to the host cell surfa…
JOHN H KEHRL · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2021 · $51,546 — Beta-Coronaviruses are a family of positive-strand enveloped RNA viruses that includes the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Much is known regarding their cellular entry and replication pathways, but their mode of egress remains uncertain. Using imaging methodologies and …
paolo lusso · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2021 · $121,121 — The project has yielded the following results: 1. Synthesis of tail-modified SARS-CoV2 envelope spike (S) mRNAs to facilitate VLP formation with lentivirus core proteins. In order to facilitate the assembly of VLPs containing lentivirus (HIV-1 or SIV) core proteins, we have designed S proteins modif…
William Copeland · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · 2021 · $453,829 — During this fiscal year we continued to devote major effort to work aimed at applications of molecular dynamics and quantum mechanics/molecular mechanics simulations required to help support the computational chemistry and molecular modeling needs of NIEHS scientists. Some projects involved creatio…
Robert Petrovich · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · 2021 · $1,629,843 — The protein expression facility (PEF) functions as a support group for the principal investigators at the NIEHS. Our focus is to provide the research groups with a means to generate the proteins they require so that they can perform their experiments. These projects range from generating enough o…
Jason Matthew Elinoff · CLINICAL CENTER · 2021 — In FY21, we determined the expression levels of known and putative host viral entry molecules including angiotensin-converting enzyme 2 (ACE2), transmembrane serine protease 2 (TMPRSS2), neuropilin 1 (NRP1), and DC-SIGNR (CD209L/CLEC4M) in primary human lung endothelial cells (ECs). Results were com…
Thomas Nutman · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2021 · $163,096 — In response to the SARS-CoV-2 pandemic, we leveraged our expertise in human T cell cloning to generate SARS-CoV2-specificT cell lines from nave CD4+ and CD8+ T cells of uninfected (SARS Cov2-nave) individuals. To this end, we generated S-specific, M-specific, CMV-specific (controls) human TCLs (a to…
Audray Harris · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2021 · $249,426 — Coronaviruses remain both a seasonal health burden and pandemic threat due to the antigenic variation of viral glycoproteins and the ability of animal coronaviruses to cause zoonotic infections in humans. Although conserved epitopes have been identified, there is a fundamental gap in understanding a…
Heinrich Feldmann · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · 2021 · $203,488 — (1) Animal model development: We have established the Syrian hamster model for pathogenesis studies and countermeasure development. Following intranasal inoculation of SARS-CoV-2, hamsters consistently developed moderate broncho-interstitial pneumonia, with high viral lung loads and extensive virus …
vineet n kewalramani · DIVISION OF BASIC SCIENCES - NCI · 2021 · $356,986 — Using lentiviral vectors pseudotyped with SARS-CoV-2 Spike protein, we are examining different SARS-CoV-2 variants for their ability to interact with CLEC4M, and whether this interaction facilitates direct infection of cells or efficient transfer and infection of cells expressing canonical SARS-CoV-…
BARBARA K FELBER · DIVISION OF BASIC SCIENCES - NCI · 2021 · $650,195 — To characterize immune responses in SARS-CoV2 infected persons we examined a cohort of infected persons over 10 months post symptoms onset. Elucidating the characteristics of human immune response against SARS-CoV-2 is of high priority and relevant for determining vaccine strategies. We reported the…
alexander wlodawer · DIVISION OF BASIC SCIENCES - NCI · 2021 · $208,788 — Recently, angiotensin-converting enzyme 2 (ACE2) was shown to be a functional receptor for SARS-CoV-2, enabling the virus to enter host target cells. Using structure-based rational design, a chimeric ACE2 peptide was designed by us for use as a ligand for analytical quantitation of SARS-CoV-2. We ai…
di s xia · DIVISION OF BASIC SCIENCES - NCI · 2021 · $23,607 — Lead identification. Based on the well-established principle that the S proteins carry out all the cell entry functions of CoVs, the CoV S spike were incorporated onto a replication defective HIV core, resulting pseudovirions with SARS-CoV-2 S protein expressed on the surface of viral envelop. Trans…
Christian Mayer · DIVISION OF BASIC SCIENCES - NCI · 2021 · $7,906 — This research was primarily carried out by the cooperating laboratories. Through the use of single-cell BCR cloning and other techniques, antibody responses were characterized in patients with a history of SARS-CoV2 infection, Hepatitis B virus (HBV) infection / vaccination, as well as in animals in…
Lawrence A. Tabak · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · 2021 · $957,584 — Our lab studies the structure and function of GalNAc-Ts. We published a methods paper in Glycoconjugate J. on mapping O-glycans using solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation. This work utilized O- p…
KOEN K VAN ROMPAY · WEILL MEDICAL COLL OF CORNELL UNIV · 2021 · $217,604 — ABSTRACT – Overall Nearly 600,000 new HIV infections occur yearly in adolescents/young adults (ages 15-24 years), the only population in which HIV infections continue to rise. The end of the HIV/AIDS epidemic will be achievable only when an effective vaccine regimen can elicit long-term protective i…
Alexey Zakharov · NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES · 2021 · $184,902 — Phage display is one of most effective molecular diversity techniques, it has been used for the selection of antibodies from diverse libraries. Several facilities (Uchicago, Utoronto, UCSF) in the world have built automated phage display robot for generation of conventional antibodies to the proteom…
PAUL T WINGFIELD · NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES · 2021 · $1,638,558 — In uninfected cells, RNA is transcribed from DNA, processed, and then transported out of the nucleus and translated into protein. In cells infected with HIV-1, the viral RNA genomes must be exported out of the nucleus without being processed so they can be packaged into new viral particles. To do th…
Bernard R Brooks · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · 2021 · $1,048,765 — Migration of CHARMM to GPUs Graphical Processing Units (GPUs) are modern processors with the ability to support the concurrent execution of thousands of threads at the same time. In this work we have redesigned the CHARMM codebase from a heterogeneous CPU-GPU architecture to a GPU-only architecture…
GREG WITHAM · ANP TECHNOLOGIES, INC. · 2021 · $19,727,817 — The extreme severity of the current COVID-19 outbreak represents the single worst outbreak in recent years. Exacerbated by the fast moving, highly contagious, asymptomatic disease progression and high mortality in vulnerable populations, particularly those in low resource areas, the need for a rapid…