Document text
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FD-302 Rev. 10-6-95! __ _
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FEDERAL BUREAU OF INVESTIGATION
ALL IIJFCIITEJ-IALTIDIII CUHTAIIJEI3
HEFEIII-I IS UIJIILZFJ-JSIFIEDDATE l2l22DDEi Bl GU33-4 UE B}%K:P.fS Date of Umscription O2 23 2006
| of b- all[:::::::]socia1 security umber Q@§g§Ei~éF:EQ§%m§l ed
§%eEe.e.e;snY I*Fe"@i¢a -218WW1tu¢ee9mm.em.%;Qsewsi§e%-was 1ntervf§W¬E5*Fegyuafy'22, 2006. After bein advised of the
identity of the inte viewing agents and the nature of the
interview,[::::]provided the following information:
I was asked about an aerosol exposure performed on
I lcould not explain why there was no aerosol
exposure sheet for that particular exposure. It is possible that
either the aerosol e osure sheet was never taken out of the suite,or that it was acc3d:¬tally filed in another binder.
RUCE VINS would commonly provide Bacillus anthracis Ba! spores fol use in aerosol challenges.[::::gbelieves that when
IVINS would p epare Ba spores for a challenge, e would bring over
enough material for a specific aerosol exposure or set of ex osuresand that the bulk of Ba s ores would be stored in the| || Wsuites in building| |
[::::]was aware that a large batch of Ba Ames strain
spores were produced at Dugway Proving Grounds DPG! for use in
anthrax vaccine research and development.| |indicated that, for
the purposes of| |Ames spores were Ames
spores. It did not matter to them if they were produced at DPG or
USAMRIID.[::::]would not know where the Ba Ames spores being used
in a given aerosol exposure were produced.[:::]does not know at
what time point IVINS switched from using Ba Ames spores produced
at USAMRIID to Ba Ames spores produced at DPG.
[::::]was shown four ! copies of photographs of a one
! liter flask labeled, "Oct97, GLP Ames Spores, Left 160ml-4/21,
7737 RMR 1029," by SA| ldoes not recall ever
seeing the flask in Bui ing 1412. T e copies of the photographs
are maintained in the corresponding 1A envelope.
_|:|L"=@»
|W%@wmon O2/22/2006 m Frederick, Maryland
File# 279AWF222936USAMRIID ~ iS0;92 Date dictated N/A
SA
by SA' i§ZLE;;This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;
it and its contents are not to be distributed outside your agency.b6
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F6302 Rev. 10-6-95! '1 I I1- I ' I I
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FEDERAL BUREAU OF INVESTIGATION
ALL IIJFDRI-LTJITEEIN EIIIIIITAIHED
HEREII-I IS TJICICLLSSIFIEE _
D-'§§I'E 1.2-12-EEICIS ET 51353211 UC BAU_fDE¬_»"P.fSDate of transcription O 3 Z O3 Z 2 O O 6
On March 3, 2006, d te of birth Egc[::::::::]social security number was interviewed at
lp ace of employment, the United States Arm Medical Materiel
Development Activity USAMMDA!, B ' ' rt Detrick,
. Maryland, work telephone number After beingadvised of the identit of the interviewing agents and the purposeof the interview,[::::f]provided the following information:
|:|has | ldegr<=-e in|:| and beganemployment at the Unite States Army Medical Research Institute of
Infectious Diseases USAMRIID!, Fort Detrick, MarvlandI I
I I Iwas vaccinated against Bacillus anthracis Ba! in I
originally did not recallI I
The interviewing agents then showedI Ia
spreadsheet, whichI Irecoqnized as anl I
A copy of the shown toI His maintained in the 1A sectioJ or Ehe file. I ' rl
30% I
Investigation on 03/O3/2006 at FOI12 D¬'CIiCk, Maryland
Hh# 27 - ~ - y ~ Dmemmd N/A
SA
by SA F 92/
This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;
at and its contents are not to be dtstnbuted outside your agency. 4
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Z.FU-302a Rev. 10-6-95! _ ' 0f I
279A-WF-222936USAMRIID
hmmmMn&FDN2M __l:::::::::::::] ,OnO3/O3/2006 ,m¢
briefly lefi:ihf]interview to search [::]computerfor any RMRrelated files. returned with 15 pages of RMR
data. However, all data was taken di ctly from the sameinformation shown tol iby the interviewing agents,
USAMRIID office was
To the best of[::]recol1ection,[:::::]never entered any
hot suites.
[:::::]initia1ly believed [::;;::]physically counted thesamples and verified their location. e interviewing a ents then
section of the file. The interviewing agents discreetly showed
RMR 1029 on the spreadsheet, listed as stored in BuildingRoom[::::::::::]remarked that Room[::;]was art of theBacteriology Section and that, to the best o [:::fE:]recollection,
ad never been into any Bacteriology rooms. It is possible
went into Roon1[::] to verify the location of RMR 1029, but
odian of RMR 1029 BRUCE IVINS would most likely have toldcust , '
the location of RMR 1029. would have taken IVINS
an probably would not double check IVINS truthfulness.
I lbelievedl lstored ' theLlaboratories, one of which was in Buildir@;[:::] Room L
*6advised the interviewing agents that[:g]would b7
search computer for additional files of RMR-relate information8
WOI
I1'!?showed£::;::]a copy of USAMRIID floor lans for Buildinggmmmmu A. b7@copy o t e floor plans shown to[:::::is maintained in t e 1A b2
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h iF0-soéa Rev. 10-6-95! I, r 0
2'79AWF222936USAl92/IRIID
b *5Continuation ofFD-302 of _ , On O 3 /O 3 / 2 O O 6 , Page £_ b 7 C
and would recontact the interviewing agents if[::]were successful
at finding additional files.
2u
Rev. 61-31-2003!
FEDERAL BUREAU OF INVESTIGATION
Precedence: ROUTINE Date: O3/O6/2006
_ . . ALL INFURHATIDH CGHTAIHEETo Washlngton Fleld HHWTNTSIWUASMFHW
, _ DATE 12-12-2005 B" 60324 UE BUfDKR From: Washington Field X A "Q b6
Washington Field AMX3 b7@
Contact: I I
Approved By: hf
Drafted By: b7c
Case ID #: 279AWF~222936USAMRIID Pending!-92$S92
Title: AMERITHRAX;
MAJOR~CASE 184
Synopsis: Report results of the investigation regarding
locations the Qges strain of Bacillus92anthracis Ba! was stored,
at the United States Army Medical Research Institute of
Infectious Diseases USAMRIID!, Building 1412 and Building 1425,
Fort Detrick, Maryland. b6
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investigators interviewed persons Known to have Q
a es in eir possession during that time. Individuals were
chosen if the Ames in their possession matched genotypic and
phenotypic characteristics of those of the Ba found in the
anthraxlaced letters. References made to the "hot side" refer
to a| |containment area in the building b6
where infectious microorganisms were being handled and contained, b7@
and where special protective equipment was required to reduce the bgrisk of exposure. The "cold side" refers to the remaining areas bhof Buildingg;;;;:Lincluding areas where there is little or no
risk of exp o infectious materials, as well as some BSL3
laboratories. The location concern in Buildin were:room hot side!, suitei | cold side!, rooml I hot side!,
hot side!,| _ |
h The locations of concern in Bui inwere: sui es suite] | suite[:::] suite[::::::%:::]
Information was collected from the following eight
persons via interview and/or review of FD302's from priorinterviews: | USAMRIID!,Ltn | b6 USAMRIID!, | USAMRI , bii
U ,| | US ,
|:|¢z¢$@ W, I:
To: Washington Field From: Washington Field
Re: 279AWF222936USAMRIID, O2/28/2006
USAMRIID! Brucejg. Ivins USAMRIID!,and | USAMRIID
[:::::::]re ving seen[::::::::;g;:]in roomlg::]the walkin[gggler*jE;iff:ffcould not pro e Ormation oumaterials in but mentioned thatTLd
recalled that stocks of a ents were ke t in9 P
no provide specific information regarding Ames.
also noted that Ames was not stored in the hallways of
Bui ing 1425K
E::::::].reca11ed there being a freezer containing Ames ini1dingZ| next col |stated Ames was there for many years prior to
1999 and u to the present, including samples belon ing to[:::;;:::%E§EE%id not store an sam les in room the walkincoo er. stated Buildingi;::;i rooms| | containedonly the Pasteur strain; the on y es u here was from themailin s. stated Ba was stored inEif:rprior to 2001, used
and the Diagnostic Services Division, whoWmper orme ex ractions?
[::::%E%fjated Ames from Ivins' laporatory was notstored in room the walk in cooler. stated that there
were no freezers or refrigerators that contained Ames in the
hallways of Building 14253. 9
[::::] stated room[:::]was used asla re and post
exposure animal room for aerosol chal1enges.| |did not store
any material in romn[:::] the walkin cooler, although| |
leftover material from aerosol challenges may have been store
there.[:::::]noted that tored Ba in a freezer in the hot
hallway of Building
[::::::::::::]could not rovide specific informationregardin Ames storage in room[:?:] the w 1kin cooler but
did be1ievedi::::::]nay have stored Ba there. '
perform Ba sprays in Building [:::] Suite while Building
1412 was down for renovations possibly up until late 1999!?
[:::::::]did not store Ames in Building 1412, room[::::]
[::::::]cou1d not provide any information about Ames actually
being stored in any of the other locations in questioni
Information from Ivins was gathered from a review of
FD302s from prior interviews. Ivins did not recall being inroom[::] the walkin cooler. No other information was available
regarding the storage of Ames for the locations in question?
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- To: Washington Field From: Washington Field
Re: 279A-WF222936USAMRIID, 02/28/2006
Information from[:::::::]was gat om a review ofFD~302's from prio ' terviews. In generalEfff:ff::]could not b6identif the roomsT:i worked in with ATes_Thile in Building has' not tggall_heing in room the walkin cooler;
did not know where the Ames
used came from. never worked in a BSL4 suite andwould therefore not have used Building 1425, suite[:::j]
, The attached chart provides a summary of the above
information, gathered from interviews and review of FD-302's.
Attached hereto and considered part of this document is the above
mentioned chart.
FD302 of Oct.l7,2005! POI Serial 1483
. FD302 of Oct.l7,2005! USAMRIID Serial 1426 ,. FD302 of Oct.19,2005! - USAMRIID Serial 1425 ESQ. FD-302 of Jan.3,2006! - USAMRIID Serial 1490
FD302 of | Jan.l2,2006! - USAMRIID Serial
79Review ofggggggggggggggkffrent FD302'sFD302 of Nov.7,2005! USAMRIID Serial 1433
Review of Bruce Ivins current FD302's
= Q0
3
ALL IiriE"UF1EieTIZI§1I CiEl1ZT£tI?i'j§l
lEFLEIitI IS T§l~lCL5.iiSIFIEE1
| No info Just plates info5.1% ygtlgsggigg Be E5334 gr; 5gW;;i~_p:-;»~;=; Investigators known to have Positive Samples and Storage Locations
Hallways
No No m 0' Stocks of
agents kept;
no info on
Amesi WW 9 1412 __-MW. I I 1 1425 ;_
M] Ronni | Hot hallways Suitg I Suitei_j Suite] [_-___§uite|Noinfo No info ' fj No info No infoNone stored
inhallways
No info_|t
I
J No info WNo info
No infoDid not
store any
samples
there
No Ames
from Ivins
laboratoryFreezer with
Ames next to
Ames more
for many
years prior to
1999 and up
to present;
samples from
No infoNo info Pasteur only
agent used
there; only Ba
was from
mailingsBa stored
there prior
to 2001;DNA
extractions
done by
DSD; Ba
fromNo info
No infoNo info K 1No info No info No info There were
no idges or
freezers that
stored Ames
inhallways
Used as a
pre- andpost-
exposure
animal
room for
0 aerosol
challengesNo infoFlare not
store
anything
here; may
have been
leftover
material
from aerosol
challenges
stored herefreezer in the
hallway| ta info
store amaNo info No info No info name No info
No info No info No specic
info; if BaNo info No info No info No info Performed Ba
sprays there1
s.»
Stored p
This chait is a sunnnaiy of the infomiation gathered from the FD-302s. Revised 02/22/06
No info: person not able to provide an account of what was stored in the location.
no information to be found; nothing reported about the location.
1. 279A-WF-222936-USAMRIID -1483
2. 279A-WF-222936-USAMRIID -1426
3. 279A-WF-222936-USAMRIID -1425
4. 279A- WF-222936-USAMRIID4490
5. 279A-WF-222936-USAMRIID -1479
6. Review of prior FD-302sW. 279A-WF-222936-USAMRIID -1433.Review of prior FD-302s
X
in
' 2
Y~ we . ~ __. beNothing T No info No info No info No info No info No info No info No info No info
Ivinss No -an nu nu
t
t 1; ,,
O 1 t ¬
FD-302 Rev. 10-6-95!
_ 1 _
FEDERAL BUREAU OF INVESTIGATION
ALL INFCIHI-IATEDN CUZUTAIIJED
HEREIN I T,TI1§llIL§lSSIFIED
DATE 12-12-2005 BY 50324 UE B.!1JZxT.*IJPI,fP!.Y5 Date of transcription {O7 {2Q
On March 7, 2006,| date of birth
social security numberl home address of be
was interviewed at WC
place of employment, the United States Arm Medical Research
Institute of Infectious Diseases Porter Street,
Fort Detrick, Maryland, telephone A.ter being advised
of the identity of the agents and the purpose of the interview,
E::::]provided the following information:
confirmed tha employed bg
since in the Division b7C
suites |,in Buildin |with
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The room contained several pieces of equipment includin
a oo , s akers, and incubator_s_,J
e Iermentor was used in worK wiEH'aEtenuated strainso Baci us anthracis Ba!.[:::::]advised that there was a freezer
in suites[:::::1and that there were several -70 degree freezers inthe hallways which everyone had access to.[:::::]also was aware of
Investigation on Q3/O7/2006 at FOIt Detrick, Maryland
File # 2'7 AWF222 6 USAMRIID~92BQ_5 Date dictated
PIb 6by PI b7C
This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;it and its contents are not to be distributed outside your agency. 1
_ L?-&~ n
O I O
FD-302a Rev. 10-6-95!
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279AWF222936USAMRIID
Continuation ofFD-302 of A I , On O 3 / O '7 / 2 O O 6 , Page 2
In ZUU1J Bid not keep an inventory of organismsI lmaintained approximately a dozen spore re s
and JD to 4U seed vials. In discussing the walkin coolers,
described them, particularly during the renovation of suites
as very cluttered and that employees stored a variety of dea -
animals, plates, test tubes, centrifuge rotors, and flasks there.
92
lrecalled obtaining Amesi from a couple of vi ch coworker| Ihad maintained in a
box in the freezer. also recalled obtaining a few millilitersIof Ames from IVINS H
explained that nobody qoes into someone else's spore stock without
their consent.|
[:::::]was shown a photocopy.of a version of the Reference
Material Receipt RMR! pertaining to USAMRIID Part Number 102$,
descri e as ig l urified Bacillus anthracis Ames spores, datedOctober 22, 1997.E¬fE:]indicated thatE::;was not familiar with the
term 1029 or Dugway Spores. obtaine the document simplybecauseL:;Lhad earlier observedi Imaintain a similar|documen I |notebook.| H
The RMR receipt copy shown to] |is maintained in the 1A section
of the file.
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FD-302a Rev. 10-6-95!
279AWF222936USAMRIID
Continuation of FD-302 of | I , On O 3 / J 7 / 2 Q Q 6 , Page 3
[:::%:]recalled that IVINS maintain upply of Ames ina walkin coo er in suites[:::::]of BuildingT§:i:fand that this was
common knowledge.[:::::]was shown a copy of the floor plans for -suitesl I BuildinqJ___________1indicated on the floor plfn that
inidentified the following areas and labeled the floor plans as: cold
roomsl|The USAMRIID floor plan copy shown to
is maintained in the 1A section of the file.
J The bigger autoclave was primarily used by the
veterinarians an the smaller autoclave used by the researchers.
Usually it would take an here from 1.5 to 2.0 hours to completethe autoclave process.Ei§E:]explained that the common practice
adhered to by employees using the autoclaves was to place materials
in the autoclave and only when it was full was it turned on. Sincethere was no set policy that was followed,L;::::JeXplained that
materials were often left together on the oor from various
employees! by the autoclave waiting for it to be full before it was
run.
During a brief tour of the[::::]cold side of Building[:;;:::::g::lpointed to some flasks on the floor by an autoclave and
in icate t at some of the flasks] I awaiting to be
autoclaved.| HR Iacknowledged that
it would be wrong for a researcher to remove material from the
autoclave or autoclave area that did not belong to them.
[:;::;]was given an opportunity to review four photocopies
of one flas , rom different anglesfwhich was labeled "RMR 1029GLP Ames Spores Oct 97". After viewing the photocopies,g:::::]could
not recall seeing or handling the flask but acknowledge that the§andwriting depicted on the photocopy appeared to be that of IVINS.
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279AWF222936USAMRIID
Continuation of FD-302 of I i , On O 3 / O '7 / 2 O O 6 , Page -4-
The above photocopies shown to[:::::]are maintained in the 1A
section of the file./-Y;
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I . 2" , FIEPEIDI I5 Ul':ELa!1§§§IFIEI3-ALL IIIIFUFI-IATIDIJ CUNTAII-TED
R¢v_Q1.31.2Q03! DATE l2l2E:00I3 Bl 50324 U0 BATH.-*"DH;%
FEDERAL BUREAU OF INVESTIGATION
Precedence: ROUTINE Date: O3/29/2006
To: Washington Field Attn: AMX3
Inspection Attn: IIC[::::::::::::::] no encl.!
Counterterrorism Attn: WMDOU, SSA[::::::::::::] no encl
From: Washington Field
AMX3
Contact: I I
Approved By: I I
Drafted By: I I
Case ID #: 279AWF222936USAMRIID Pending!-92S1Q,
Title: AMERITHRAX;
_ Major Case 184;
OO:WF
Synopsis: A summary of the results of two initiatives, each
whose goal was to determine how correctly and consistently
keycard access badges were used by employees who were involved in
aerosol challenges in Building 1412 of the United States Army
Medical Research Institute of Infectious Diseases USAMRIID!,
Fort Detrick, Maryland.
Enclosure s!: 1! Six ! page copy of keycard data titled
"September 16, 1998 Aerosol Challenge," 2! Six ! page copy of
keycard data titled "September 14, 1999 Aerosol Challenge," 3!b6
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Three ! page copy of keycard data titled "October 15, 1999Aerosol Challenge." I
Details: Efforts were made to obtain a general idea of whether
keycard access badges were used correctly and consistently byem loyees who were involved in aerosol challenge work in Building §§F[;;:]at USAMRIID. Two separate initiatives were conducted to
1S end, each described below:
1. Examination of the use of the five main access points to andfrom the containment area of Buildingg for the days of and
davs after three aerosol challenges. 92K
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Aerosol challenges were conducted within a BiosafetyLevel 3 containment area in BuildingI::::] Proper entrance into
I:IO/0306' 66- I:
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To: Washington Field From: Washington Field
Re: 279AWF222936USAMRIID, 03/29/2006
For
access points
an individualthe purposes of the initiative, each of the five
was assigned a number as it is labeled above. For
who entered and exited the containment area ofb2
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Building 1412 using all the required access badge readers, the
keycard access records would typically follow the order 1!, 2!,
3!, 4! and 5!, with other keycard entries possibly appearing
between 1! and 2!, 3! and 4!, and 4! and 5!.
Keycard access records were examined for 9/16/1998,
9/14/1999, 10/15/1999, and the days after each of these three
dates, The abovementioned dates were chosen because they were
possibly the first three times RMR 1029 was used in aerosol
challenges at USAMRIID. The days after each were included
because postchallenge work, such as the culturing of Bacillus
anthracis Ba! from the allglass impingers, was most likely done
then.
For 9/16/1998 and 9/17/1998, the keycard access records
of the following individuals were examined due to their mention
as bein involved in the challen 'th th h 2 g e ei er roug Db
sheets or in interviews:| I BRUCE921V b7c
| The keycard records of
were also examined for these dates, in order to compare
access to that of individuals that were known to be in t e
containment area of Building 1412.
For 9/14/1999 and 9/15/1999, the keycard access records
of the following individuals were examined due to their mention
as being involved in the challenqe, either throu h aerosol datasheets qr in int§rview§;J q_|BRUCE IVINSrThe keycard records of[:::::]
were also examined for these dates, in order to compare
access to that of individuals that were known to be in the
containment area of Building 1412.
For 10/15/1999 and 10/16/1999, the keycard access
records of the following individuals were examined due to their
2
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To: Washington Field From: Washington Field
Re: 279A-WF222936USAMR.IID, O3/29/2006
mention as being involved in the challenge, either through
aerosol data sheets or in interviews | b7?
BRUCE IVINS,| | No keycardaccess records forl Iexist for these dates.
The keycard access data for the abovementioned dates
and individuals are enclosed with this communication. Data are
presented in a spreadsheet, with relevant access points labeled
by number on the righthand side of the page. Also included are
the highest total numbers of access points recorded at one time
out of five! for each date and each individual whose records
were examined. This number demonstrates how many of the five
relevant access points were used in the correct order for entry
into the containment area on the given date. For several
individuals, there are also extraneous entries into ! and exits
from ! Building 1412.
For all of the days when access points were recorded
for employees who used the containment area to work with aerosol
challenges, at least three of the five access points were
recorded together. There was therefore always some indication
that the employee used the change room or keypad to enter the
containment area. In approximately 50% of the cases, one or two
of the access points were missing i.e. 3 of 5, or 4 of 5 points
recorded!. For the other approximately 50% of the cases, all
five access points were used correctly i.e. 5 of 5 points
recorded!. Never on a day when an employee was assigned to work
in the containment area was he/she shown only to have entered and
exited the building on that da i.e. 2 of 5 points recorded!.Of the dates examined,[:::::::E:]keycard access records include
relevant access points on only one date, 9/14/1999. On this
date, only two of the access points are recorded, 1! and 5!. Inthis respect, [;;:::::::lrecords are inconsistent with those of
the employees w o were nown to have used the containment areas
on those dates records show no relevant access pointson 9/16/1998. Through the analysis described above, it istherefore found to be unlikely that[:::;::;]used the containment
area of Building 1412 on 9/16/1998 or 9 14 1999.
2. Examination of the use of the three main access points into
and out of the containment area of Building 1412, for aerosol
challenges between 9/14/1999 and 8/14/2001.
For each aerosol challenge that occurred between
9/14/1999 and 8/14/2001 and is listed on the spreadsheet entitled
"Aerosol Data Sheets, Anthrax Protocols, 12/1996 through 8/2001,"
the keycard access records for each Principal Investigator PI!
and aerosol technician tech! listed were analyzed.
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, To: ,Washington Field From: Washington Field
Re: 279AWF222936USAMRIID, O3/29/2006
The three keycard access points of concern were 1| Entry into the male or femalT change room via badge swipel:::E::j2! Entry into the containment areavia keypad codd kEYPAD"!H|Exit from the male or female change room via badge swipe
These access points are
referred to on the below chart as 1! IN, 2! KEY and 3! OUT.
The following chart describes each employee's keycard
access records for each date they were listed as having been
involved with an aerosol challenge using Ba. Data in the fourth
column are recorded as follows: "Yes" indicates all three
relevant access points were recorded in the proper order IN, KEY
and OUT!, "None" indicates none of the relevant access points
were recorded, and any anomalies or missing access points are
mentioned.
Use of the Kevcard System in Building 1412, Rooms[:;] and[:::]bv PrincipalInvestigators and Aerosol Technicians During Aeroso Chal enqes Between E
g/14/1999 and s/14/2001
Exposure# Date PI/Techs IN/OUT/KEY consistently?
As
9/14/99
10/15/99
4/5/O0
4/7/O0
4/10/O0
7/l7/O0
7/18/00Yes
IN/KEY but no OUT
Yes
Yes
None
One extra IN, all else okay
None
Yes
Yes
None
Yes
Yes
None
Yes
KEY/OUT but no INb2
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To: Washington Field From: Washington Field
Re: 279AWF222936USAMRIID, O3/29/2006
k2/5/00 Extra OUT between IN and KEY
Yes
YES bg
B. Ivins Yes b7C
IN/KEY but no OUT
Yes
Extra OUT or KEY/OUT but no IN
IN/OUT but no KEY
None
Yes
E2/7/O0 None
Yes
Yes
B. Ivins Yes
IN/KEY but no OUT
Yes
Yes
Yes
Yes
KEY but no IN or OUT
E2/11/00 None
Yes
Yes
B. Ivins IN/KEY but no OUTYes
Yes
Yes
Yes
>* x
TO:
Re:{i i uWashington Figd From: Washington Field
279AWF222936USAMRIID, O3/29/2006
4/12/Ol
6/26/Ol
7/10/O1
7/ll/01
7/12/O1
8/14/01None
Yes
Yes
Yes
Yes
Yes
Yes
Yes
I, -To: Washington Figd From: Washington Field.
Re: 279AWF222936USAMRIID, O3/29/2006
Anomalies indicate that the employee may have
piggybacked through a missing access point with another
individual, or the system might not have read a keycard swipe or
keypad code properly. "IN and OUT with no KEY" is most likely an
anomaly if there is a significant time lapse i.e. several
minutes! between IN and OUT.
The amount of anomalies evident on the spreadsheet
imply that there were most likely instances of piggybacking
and/or system faults through use of the change rooms into the
containment area of Building 1412 on these dates. I |
stated durinq an interview thatl I
| the ack of KEY accesspoints for| _ _ lentered the
containment area through one of the abovementioned means.
Additional review and interviews could be done to determine how
many of the above individuals may have piggybacked or experienced
keycard system faults when entering and exiting the containment
area. Individuals whose keycard records show anomalies or no
access points at all could be further investigated, and a study
could be done of the history of failures of the keycard access
system.
The initiatives described above demonstrate a
significant difference between expected keycard access data for
employees using the containment area of Building 1412, and the
actual data for employees during several Ba aerosol challenges
between 9/1998 and 8/2001. The records of these employees often
did not include at least one access point that is required for
entering or exiting the containment area. The initiatives
therefore have demonstrated that it is likely employees involved
in aerosol challenges have piggybacked and/or experienced system
failures when using keycard access badges and keycard access
codes. All records in one initiative did show at least some
indication of the use of a change room, however; it is therefore
unlikely that an individual who worked in the containment area
during an aerosol challenge would have access records that show
no access at all to one of the change rooms.
Q0
7 ,b6
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" I
September 16, 1998 Aerosol Challenge9/16/98
9/16/98
9/16/98
9/16/98
9/16/98
I
-
9/ 1 6/98
9/16/98
9/16/9818:00:02 Access Granted
18:00:36 Access Granted
18:1 1 :10 Access GrantedSeptember 16, 1998 Aerosol Challenge
Normal
Normal
Normal9/17/98
9/17/98
9/17/98
9/16/98
9/16/98
9/16/98
9/16/98
9/16/98
9/16/98
9/16/98
0
9/16/98
9/ 1 6/98
9/ 1 6/98
9/16/98
9/16/98
9/ 1 6/98
9/ 1 6/98
9/16/98
9/ 1 6/98
9/ 1 6/98
9/16/98
9/ 1 6/98
9/16/98
9/ 1 6/98
9/ 1 6/98
9/ 1 6/98
9/ 1 6/98
9/ 1 6/98
9/16/98 Access Grantetl _ln_
9/17/989/17/98
9/17/98
9/17/9816:28:56 Access Granted
16:30:35 Access Granted
16:31 :32 Access Granted
16:32:33 Access Granted
16:33:33 Access Granted
16:35:14 Access Granted
16:35:54 Access Granted
16:37:19 Access Denied
16:38:37 Access Granted
16:40:08 Access Granted
16:41 :00 Access Granted
17:56:44 Access Granted
17:58:18 Access Granted
17:58:51 Access Granted
18:04:46 Access Granted
23:54:33 Access Granted
23:55:22 Access Granted
23:57:00 Access Granted
0:20:17 Access Granted
0:20:43 Access Granted
0:22:28 Access Granted
0:23:03 Access GrantedSeptember 16, 1998 Aerosol Challenge
Normal
Normal
Normal
ln
Out
In
Out
lnNormal
Normal
Normal
Normal
ln
Out
u9/16/98
9/16/98
9/16/98
4 Q9/17/98
9/17/98
9/17/98
'3 I
9/14/1999
9/14/1999
9/14/1999
9/14/1999
9/14/1999
9/14/1999
9/14/1999September 14, 1999 Aerosol Challenge
7:31 :48 Access Granted
7:32:19 Access Granted
7:36:17 Access Granted
14:05:16 Access Granted
14:06:31 Access Granted
16:51 :50 Access Granted
16:52:-16 Access GrantedNormal
InNormal
Normal
Normal
Normal
9/14/1999
9/14/1999September 14, 1999 Aerosol Challenge
14:51 :30 Access Granted
15:33:23 Access GrantedNormal
Normal9/15/1999
9/15/1999
9/15/1999
9/15/1999
9/15/1999
9/15/1999
9/15/1999
9/15/1999
9/15/1999
9/15/1999
r9/15/1999
9/15/1999
9/15/1999
9/15/1999
A 0
9/15/1999
9/15/1999
9/15/1999
9/15/1999
9/15/1999
9/15/1999
G
v n9/15/1999
9/15/1999
9/15/1999
w-1~-M
10/15/1999
10/15/1999
10/15/1999
10/15/1999
10/15/1999
+ a
931?t Ya
10/15/1999
10/15/1999
10/15/1999
10/15/1999
10/15/1999
10/15/1999
10/15/1999October 15, 1999 Aerosol Challenge
13:31 :41 Access Granted
14:17:40 Access Granted
14:18:05 Access Granted
17:43:32 Access Granted
17:43:57 Access Granted
17:48:24 Access Granted
17:48:36 Access GrantedNormal
Normal
Normal
Normal
4.
1 | Us10/15/1999
10/15/1999
10/15/1999
if
._ ,
FD-302 Rev. 10-6-95!
-1-
FEDERAL BUREAU OF INVESTIGATION
ALL II-IFUPHATIUI-I EDI-ITAIHED
PEPEIIJ IS UI~]CL.i'iS5IFIE[3
DATE lZl22lIlEl6 BY 503241 UC BAIIT,-DE.-"R'£'5 Date of transcription Q 3 Z 2 2 f 2 Q O 6
On March 20, 2006,]I I social security numbedate of birth
ome address of ft viIwas interviewed at[::]
place of employment, the United States Army Medical Research
Institute of Infectious Diseases USAMRIID! Porter Street,
Fort Detrick Mar l d t l h Aft b d d ' , y an , e ep one[%;::::::::£i::] er eing a vise
of the identity of the agents and t e purpose of the interview,
[::::::]provided the following information:b5
b7C
' confirmed that| Ihas been employed in the
Division at USAMRIID since
Iis more commonlv referred to asb6
b7C
as never metl rbut recafled one occasion
aving a te ephone conversation with| I
ll Ti mt 92nD.J:k.@<'1 di::;ectl_v
I S in the ast.
ha
materials. There has not been any dry or
at USAMRIID since 1972. |:|does not know
[:]thou ht that Aberdeen Proving Groundswork. E§::::]did not observe any unusualrecalled IVINS telling[:::]an individual
stock of Bacillus anthracis Ba! from 8s only worked with liquid
offensive work performed b;
anyone who does dry work. b§C
may be involved in dry bg
activity at USAMRIID. [:1 b7F
could obtain a usable
liter flasks. It was common
knowledge at USAMRIID that Ba Ames was stored in the[::::] suites
of Building 1425. The cold rooms in these areas were not locked.
Anyone could obtain materials from the w
coolers was based on the "honor system".
walkin freezers. Freezers were kept outalkin coolers; use of the
The suites did not have
in the hallways. Current
handling procedures involving select agents have been significantly
modified in that the use of select agent
and individual accountability maintaineds must now be documented
in a database.
Investigation on March 20 , 2OQ!6 FOI11 D¬tIiCk, Mary land
k# 279AWF222936USAMRIID'Y§5l
PI
by PIDate dictated
b6
b7C
This C|OCUment contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;
I
92
>
92
1b 6Continuation ofFD-302 of ' I , On March 2 Q , 2 0,Q6gc 2 b *7 <FD-302a Rev. 10-6-95! '
279AWF222936USAMRIID
[::::::]volunteered[:::]thoughts concerning the anthrax
powder contained in the mailings and explained that investigators
should be focusing on the chemistry of the anthrax. [::]explained
that spores normally tend to stick to each other but with the
anthrax powder, the spores had to have been separated. In order to
accomplish this, a positive or negative charge had to be
introduced, a hyperphobic or polar molecule, like a dry detergent,and theT:?ifled during growth. The material must be charged and
polar. believes that the use of a mass spectrometer would aid
in identifying the chemical make-u of the anthrax. indicatedthat if asked,E:] would volunteerE%:] assistance inho
b7C
Iexplained
that room housed a 100liter fermentor known as "Big
Bertha"] Ithere were two sep r 100-liter
fermentors known as "Big Bertha" in Building| | USAMRIID
originally obtained these fermentors around 1980-19851 I
The 100-liter fermentorsk] Iwere
could best provide a historical perspective of the fermentors.
[:::::::]explained that fermentors were primarily used backin the 1980's but thef:jse drastically declined due to the advent be
[iiiiiifof molecular cloning advised that a bioreactor is similar to a b7c
or and that it is possible to grow Ba using a bioreactor M
was given an opportunity to review a document titled
Inventory Check and Corrections: 8 Nov 2005.
"Biq Bertha" is the 100-liter fermentor identified as stock
number| Ion the inventory sheet and believes thefermentor was purchased in 1983. ltwo Braun
Biotech fermentors, stock numbers 20 liter?
1 r92n Q n.
FD-302a Rev. 10-6-95!
279AWF22293 6USA192/[RI ID
Continuation <>f1=n-302 of I I ,on March 2 O , 2 O,Q6ge 3
1:-6riknmmmxm and] L10-liter fermentor! as two fermentors bqcalso identified a New Brunswick, 'Bio Flow III, 3liter fermentor, stock #l::;;:::::::::]as having
been moved from suitel |Durinq ur er review of the
Inventory sheet shown to is
maintained in Ehe IA section of the file.
During significant segments of the interview,[:::::::]
asserted that the Anthrax investigation was not focused in the
right direction; that the investigative spotlight should be much
broader and that investigative efforts should focus more on the
human/financial motivational aspects of the crime as opposed to the
scientific angle. [::]suggested that investigative efforts should
be more focused on a company called Bioport and not USAMRIID.
E:::::]indicated that the investigation has greatly interfered with
USAMRIIDs work and that a closer scrutiny of Bioport would reveal
that Bioport is the one entity that has financially benefitted the
most from the anthrax mailings. USAMRIID has not benefitted from
the anthrax mailings but in retrospect has suffered.[:] explained
that since the anthrax mailings and the subsequent investigation at
USAMRIID, funding has significantly been cut, projects have fallen
behind schedule, and the implementation of control and
ability measures has been restrictive and time consuming.[?¬f?f%iexplained that it currently takes approximately 12 years and
$800 million dollars to develop a new biological product and thatthe anthrax mailings, in[::]c@union, have caused a two year delay.
According toE::::::]prior to the anthrax mailings,
Bioport's anthrax vaccine program had been cited for 14 violations
by the Food and Drug Administration FDA!, also known as 483
violations, resulting in Bioport having to stop production and sale
of the vaccine. During this same time period, USAMRIID had already
been working on a new anthrax vaccine which was ready for clinical i
testing. USAMRIID was directed to go to Bioport and attempt to 92
resolve the deficiencies that existed with the old anthrax vaccine
p O ram. USAMRIID employees BRUCE IVINS, | I |[:§:i:::]were tasked with this effort. Bioport needed to improve its
emp oyee training program, update their facilities, and validate
their efficacy models. Ultimately, as a result of the anthrax
mailings, Bioport's anthrax vaccine was relicensed. [:::::::j
asserted that Bioport was ultimately successful in delaying the
development and sale of the new USAMRIID anthrax vaccine while
I 0 ,-
FD-302a Rev. 10-6-95!
b6
13711?
279A-WF222936USAMRI ID
Continuation of FD-302 of I I , On M8.ICh 2 0 , 2 O,Q5ge __§-_
prolonging the sale of their old anthrax vaccine.[:::::]further
claimed that there was an FDA board member who was affiliated with
Bioport. [:::::]was unable to elaborate on this.
[:::::]volunteered that, in 2000, a connection existedbetween Qynport Vaccine Corporation BVQ! and Bioport. | |
explained that USAMRIID's competitor, DERA a British government
agency, and Bioport are affiliated, I |e ' r
USAMRIID emplovee|
H::::::::]wouId have more insight into Ehis Eheory.
In attem ts to provide further assistance with theinvestigation, E::f::]opined that the facility in Boca Raton,
Florida that is believed to have received a mailing containing
anthrax may also house offices for the CRC Press which publishes
critical reviews in science and chemistry. Bioport's vaccine
program may have been negatively impacted by this company. y
On or about March 21, 2006,E::::::1provided Inspector
an email which contained the following attac -
These documents will be maintained in the 1A section of
e 1 e.
1 - '_ Rev. 01:31-2003! . 5 I
F 0
FEDERAL BUREAU OF INVESTIGATION
Precedence: ROUTINE Date: 04/12/2006
To: Washington Field ALL Irc[F|:;m:I,"I'1=:|1a cu1~r1"a,:r~11zrJ
HIIFLEIH I? TIIIICLASSIFIED ,Fr°m Washington Field nrn:12i;umuaBv anzauzsiwmm,AMX3 I
Contact: I I
Approved By:
Drafted By:
Case ID #: 279A~WF222936USAMRIIDP// Pendingydgu '
Title: AMERITHRAX;
MAJOR CASE 184 I1127C
I36
b7C
Synopsis: ' b2
review b7E
I Ifrom the United States Army Medical
Research Institute of Infectious Diseases USAMRIID!, Fort
Detrick, Maryland.
Enclosure s!: Enclosed for Washington Field are copies of the'
leads addressed in this communication, generated by the
' electronic mail review.
Details: I II rfrom the United States Army Medical
Research Institute of Infectious Diseases USAMRIID!, Fort
Detrick, Maryland. These records were reviewed by Amerithrax
investiqators and deemed worthv of further '
I but writer located and reviewed Ehe toflowinoI notebooks bvI I
Notebook Number IRelevant Paqes Found
1151 A few pages;I I
lyophilized anthrax likely
all Sterne strain! in 1978.
1214 INO
I36
I I37C1
. "TO: Washington Fd From: Washington Fiel
Re: 279AWF222936USAMRIID, 04/12/2006
1282 NO
1 1381 No
1 1399 NO
1400 NO
1435 NO
1455 NO
1523 NO
1527 No
1582 No
1587 No
1807 NO . b6l i b7C
[:::;:;::;::::] written on August 17, 2000 by Bruce' vea e t at Ivins deemed it necessary to review[:::::]
notebooks in hopes of gaining information on Vollum 1B,
1 lot 189 spores. To that end, the following notebooks by[::::::]
which were all that remained at USAMRIID, were reviewed by
1 writer:
Relevant Pages Found Notebook Number
§ 195 NO
441 No
447 No
454 NO
92
461 No
471 NO
473 NO
I One notebook, #3369, was not reviewed as it was currently in a l $6inment suite. Notebook #3793 was missin for a time,L::::] ?ZcEff??? but was found on December 16, 1997,[:::fE:;;] and la er DID
reviewed ' er. Pages from #3793 were copie pertaining to avisit by{?f:iiTforeign visiting scientists on October 17 and
2i
r
4 1 '
To: Washington F1'd From: Washington Fiel.
Re: 279AWF222936USAMRIID, 04/12/2006
October 18, 1995. Notebook #1767 was also listed by as
missing; it is unclear whether #1767 was ever locate u writerdid not locate this notebook during the notebook review. blc
Notebook Number Pages Copied
1946 No
1947 No
1948 No
1970 No
1971 No
1972 Some;|:|received Ames from
Ivins
3064 No
3065
3066One a e- mentioned new name
of
No
3184 No
3185 NO
3263 No
3264 No
3265
3343One a e- mentioned new name
of
No
3344 No
3345 No
3413 NO
3414 No
3415 No
4, ITo: Washington Fd Fr_om: Washington Fiel
Re: 279AWF~222936USAMRIID, O4/12/2006
3417 No
3418 No
3675Notebook Number Related to Anthrax
No
37101 No
3776 No
3856 No
3954 No
4026 NO
4027 NO
3772 NO
3944 No
4151 No
4279 Yes
4285 Yes
Q0
4b6
b7C
u I 92 _
FD-302 Rev. 10-6-95! a I I
. _ 1 _
FEDERAL BUREAU OF INVESTIGATION
ALL II*IFi:|E!IL?&TIi]I*I CBIJTAIIIED
I{EEtEIIi] Iii UIlIELELEiSiIFEEZ.| ' - .
DATE l2-12-ZIIIDE? Bl" ECIEIZ-ill UC EAI!.T,»"DI-I,-*'IFi'1'E? Date °f IT3"5¢P°,n 05[ O 81 2 0 O6
I I I date of birthI I social security account numberI I residing atwas interviewed atI Iplace of employment I
ItelephoneI I email address
After being advised of the identit of theinterviewing agent and the nature of the interview,[::Tprovided the
following information:
I I From
I Iwas employed as aI I at' the United States Army Medical Research Institute or Intectiousl
Diseases USAMRIID!.
employed at USAMRI ID. I I
Investigation on 05/ 04 / 2066 at279AW___F22293'6"LEAD' -Q I
- FiIe# 279A-wF-222936-UsAMRIID~/5441 Datedietated 05/os/zoos
by SAI I I
/I
This document contains neither recommendations nor eonclusions.of the FBI. It is the property of the FBI and is loaned to your agency;
. it and its gqntents are not to be distributed outside your agency. 1
- jv=9/I-/11,4-i;ll9.%»-.Z13/-*6 -' /38$be
IteIFphoneI I bc
_ 1-1
__ 1. ,FD-302a Rev. 104$-95! _ 3
279AWF222936-USAMRIID
Continuation ofFD-302 of I I , On O 5 / 0 4 / 2 O O 6 , Page 2 b 5
b'7C
While employed at USAMRIID,[:::::::]never observed any
unknown or unauthorized person working in suites B3 and B4 in
building 1425., Regarding operation of the autoclave, one
particular individual was not responsible for running the machine.
Instead, each scientist and technician was responsible for b§_
autoclaving his or her own waste, and those individuals were bQ
responsible for legibly marking the material autoclaved. [:::::::]
noted that the animal caretakers who entered the suites daily to
care for the animals being used for experimentation were
responsible for autoclaving all of the animal waste and bedding
y materials from the animal cages.
It was general knowledge that wet spore preps of the Ames
strain of bacillus anthracis BA! were made in suites B3 and B4.
The walk-in cooler which was located in the common hall that
internally connected suites B3 and B4 was a "busy" place, as it was
used by almost everyone with a laboratorv in suites B3 and B4.
| |used the walkin
cooler regularly. There were no sign-in procedures for the cooler.
The following types of things were maintained in the cooler:
reagent media, sterile media, sterile plates, buffers, innoculatedg
' plates from ongoing research, boxes of samples, and tins containing
a variety of things such as organic reagents. It was not uncommon
to find that when you placed your research material in the cooler,
another researcher might move them to a different location in the
cooler.
beAlthough| |is familiar with BA Eiology,L__]did not conduct I b7C
research regardin BA. and never-worked with BA. [:]noted that on
one occasion| Ia wet prep of BA spores beinq conductedby Bruce Ivins, but] r_________Hlfor the BA vaccine. |
h_i__
.
x 92 g FD~302aIRcv. 10-6-95! . 3
2'79AWF222 93 6USAMRI ID
b 6Continuation of FD-302 of I I , On O 5 / O 4 / 2 0 O 6 , Page 3 I97 C
is not familiar with Ames "1029" BA, but has heard of "Dugway
spores". noted that it was commonly known that Dugway made
spores, and recalled that post 9/11, there was discussion
related to ugway spores regarding the creation of a standardized
spore prep for use in the challenge studies being conducted for
vaccine testing. Regarding distribution of samples within the labs
' in the suites, the prinicpal investigator for each lab set up his
or her own policies and procedures regarding how they distributed
samples. If a spore prep were to leave suites B3 or B4, then the
USAMRIID safety office worked with the investigator to document the
move and ensure all safety requirements were met.
If dr work was being conducted in any lab in suites B3and B4,[:::::::fbe1ieves other em lo ees would have noticed, asthis would have been unusual. [::¬::E:]does not believe that a b6
lyophilizer was maintained in suite B3 or B4, but noted they did b7@
contain several speed vacs which were used for low volume drying
procedures. I
I Inoted thatIIduringI__J employment at USAMRIID. I
However,L__Jcould not recall every person who worked there.
recalled having contact with the following individuals:
Iand another individual whose
nameI Ican't recaIl;I * I IL IBruce Ivins primary 1nvest1qatorLj
rdoes not recall
any of the individualsI Iworked with making unusual observations
about other coworkers.
. 1 The working relationship among all coworkers was good,
and[:::::::]never noted any specific conflicts or tensions, withNone exception. I l Iwas having some kind of conflict with the
but[:]cou1d nothrecall what the conflict was about. I does not knowI I I
Idoes not recall ever coming across aI I
I but does
believe aI Iby the name ofI Imay have worked for
____i________________ . _ _____________________________________J
FD 302a Rev. 10-6-95! Q! .3
279AWF222936USAMRIID
b6
b7C
Cmmmmmowémof ] ] ,Q1O5/O4/2006 ,Pme 4
E:::::::::]at some p ' t.[:E;5::]noted that some of the soldiershad access to suitestij and working as assistants to various
investigators. ,Suite access depended on the primary investigators
for the different labs located in the suites.
noted the l5Oliter fermenter located in suites
[:1 and was not used frequently. There was generally little need
to produce anything on such a large scale, and the process of using
the fermenter was very messy due to the parts and supplies required
for operation. The only individuals] ]could recall
specifically who used the fermenter were] ] b5
b"C
could not specifically identify the biological agents used-
in the fermenter, but speculated that recombinant BA preps made
with an avirulent strain of BA, non spore forming, were likely
used. A virulent, spore forming strain of BA would not be used in
the fermenter because it would be a safety issue. Due to the size
of the fermenter, and the mess it created in the room where it was
located, it would be impossible to ensure complete decontamination
after use with a spore-former. The fermenter was not portable, and
was located in a room dedicated specifically for the fermenter. In
order to remove the fermenter,.a wall would have to be removed.
Approximately one year after 9/11, there was a discussion about
loaning it to a private company. The fermenter was going to be
removed and transported to the company for their own use, and in
exchange the company would use it to produce large amounts of some
recombinant proteins for USAMRIIDs use in vaccine work. However,
this transaction never occurred] ]|:| '
E::::::]is not familiar with the protocols for bacteria
grown in a fermenter, as fermentation biology is not[:::]area of
expertise. [::]is not aware of any spores from any bacteria being
grown in the fermenter. [::]is unaware of any individuals who
showed a special interest in the fermenter, discussed using it for
producing the Ames strain of BA, or expressed an interest in 'borrowing the fermenter. [:::::;;assumes that there were equipment
logs and records ertaining to t e use and maintenance of thefermenter, but[::fcan not verify this. _
Regarding the production process for the Ames strain of
BA,[:::::::]ncted it was renograffin purified, which is part of ab6
b7C
I 4 .l 1a
v
FD-302a Rev. 10-6-95! 3 7
.
_ v
27'9AWF222936USAMRIID'
b6Continuation ofFD-3020f I ,On O5/O4/2006 ,Pagc _5 b7c
wet prep purification process. [::::::]is not familiar with
renocal purification. Regarding destruction of "lots" of BA,
_ [::::::] noted that you would archive a small part of a lot, and
then destroy the rest by autoclave. [::::::]does not know if any
Ames scheduled for-disposal was recycled to be used for adhoc
- studies. [:::::::]could not elaborate on the rules that were in
place for the access, storage, and dissemination of Ames, and noted
that these rules/procedures were controlled by the individual
investigator in the lab who dealt with the bacteria. E::::::]noted
that Bruce Ivins was always very good with documentation, and was
responsible regarding distribution of samples.
vi"I P JI9 k r or
FD-302 Rev. 10-6-95!
-1-
FEDERAL BUREAU OF INVESTIGATION
ALL II-IFIIIHIIATIIJI-I EDI-TFAIHEII
HEHEIIII IS UNCLéHSIFIEII*
DA'1'E lEllZ2ClDEi BY EDEZ4 TIC EAlIIi"DH_.-"R'!' Date of transcription O4 Z 2'7 Z 2 Q O 6
On April 25,| Idate of birth bg
social security numberq I home address of b7C
Iwas interviewed at[:::]
place of employment, the United States Army Medical Research
Institute of Infectious Diseases USAMRIID! Porter Street,
Fort Detrick, Maryland, telephone A ter being advised
of the identity of the a ent d h ' 'g s an t e purpose of the interview,
Iprovided the following information:
I Iconfirmed that[::]has been em lo ed in the
Iat USAMRIID since workin in heIindicated that E1 b7@and that a majority ofI research involves the Ames
strain o Bacillus anthracis Ba!I I
[::::]reiterated that most ofI Iresearch pertains to the
Ames strain of Ba.I
Iat USAMRIID,the b@_Federal Bureau of Investigation obtained samples from all of the Ba bispore stocks contained in said freezer.I Ibelieves thatI I $§FIobtained the Ba Amfs from either BRUCE92IVIN$,I EA :/
e
§grows small batches of vegetative cells trom
original stock and believes that gr ' '
the original stock keeps the spores
Investigation on April 26 , ZUQIS FOIC D6lZIiCk, Maryland
File # 2 '7 ID Date dictated
PI
by 8A b6
b7C
This document contain; neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;it and its contents are not to be distributed outside your agency K/C 2 ~
92.n 5 $e
FD-302a Rev. 10-6-95!
2'79AWF222936U-SAMRIID b6
b7
Continuation of FD-302 of I I , On Apri 1 2 6 , 2 O,Q6gc 2
1:6
b7C
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Ba spore growth in flasks with LeightonDoi media and
usin the hot wat /shaker apparatus typically occurred over a[::::¬::]duration.T;:%fETrecalled occasional instances where, afterstarting the proce ure on Fridayq[::]returned on Monday to find the
Ba vegetative cells had not exhausted their LeightonDoi media
nutrients and therefore had not under gone sporulation, or were in
various stages of sporulation i.e. a mix of ve etative cells,cells under going sporulation, and spores!.[:::f]also noted on
other occasions vegetative cell growth was too rampant. In
situations like this, the Ba spores that were formed were of poor
quality and "clumpy".[::::]advised the ratio of Ba vegetative cells
to media volume was directly proportional to spore quality: too
much Ba vegetative cells within a defined media volume results in
poor Ba spore quality.[:::]advised it was standard procedure to
allow the Ba vegetative cells to exhaust their nutritive supply in
order to induce sporulation.
indicated that| |research does not involve dr Ba,
The s ores were never dried advised t | I P O_
knowledge there was no "dry work" co ducted at USAMRllD4rather itwas well known that Battelle was invo ed in "drv work". Typically]
indicated the aerosol challenges] lpertain only to
the use of wet or liquid spore preps.| Irecafle an experiment
id Ithat r_]was involved in that usedl I
|are more susceptile to Ba
infection, and this is advantageous in studying certain aspects ofb6
b7C
v m X. 6 '
92FD-302a Rev. 10-6-95!
279AWF222936USAMRIID
Continuation of FD-302 of I I , On Apri l 2 6 , 2 Q,Q5gc _3_ b 6
anthrax pathogenesis. This is the onl instance[::::]could recallthat[::]was familiar with in which E:Twas used.
Icould not recall which strain of Ba was
used in this particular stud at thd[::::]opined the only countryi |was knowledgeable of having the
Ames strain of Ba was the United Kingdom.92 l Irecalledl H
I
As an aside note,[:::] recalled that|
lyophilized a sam le of irradiated Ames strain of Ba on the coldside of[:::::::::¬]recalled that the Ames was "deadn" E:::]further
advised the lyophilized sample was used in antigen production.
opined these antigens were unique to the spore coat of the Ames
Strain of Ba E:::]could not recall any additional information.|:l
H has heard various stories from coworkers
pertaining to stories
that describeI r____Lhas neve met|bl/C
Fgaozawwloégs! ALL Inrnnnirrnm EUETAZNED -' ' M
HREIM I5 UHILAESIFIED
DATE 12-l223US BY @0224 UC Biwrnrr_ 1'
1; ,5 G ,
FEDERAL]HEMIIOFINVESTHHUION
Date of transcription O5 [ 18 Z 2 ! Q 6
On May 9, 2006I I date of birth
social security number work telephone number[::::] was interviewed atI Iplace of employment, the United tates
Army Medical Institute of Infectious Diseases USAMRIID!, Building
I E::::] After being advised of the identities of the interviewing
I agents,[::::::]provided the following information.
I IstartedI Iemplovment at USAMRIID i Ib6
b7C
be
b7C
b2
month Employees enerally know that Bacillus anthracisg kept in freezers in theI Isuites. Approximatel I
|Ian aerosol c a enqe in Building 1412.I I
trainin at USAMRIID. I Iwas described as ver thorou h.[:::::::jhas had only positive interactions with[:::f:::::::fIVINS.
I Iis not familiar withI _I__J II Due to I Ilimited amount of
time in theI Icou d not provide further
information about unusual lab activities or observations about
coworkers.
E::::::]is familiar with the term "Dugway Spores" but has
no further knowledge about them. [::::::] was shown pictures of a
flask known as Reference Material Receipt 1029 RMR 1029!.[:::::]
could not recall seeing this particular flask, but in general, has
seen many little flasks in the refrigerators and throughout the
labs in Building 1425.
[:::::::]is not familiar with any dry work at USAMRIID.
E::]has only worked with liquid spore preps from the AllGlass
Impingers AGIs!.
Attached to and considered part of this document are
photocopies of four different angles of the flask RMR 1029.
Investigation on O5/09/O6 at FI¬d¬1'.iCk, MD J I
m# 279A-WF-222936USAMRIID-925$92 Ihmdmmm
PIby 5| Isuites. I ' Istated| Iwas only in the suites once or twice a
assisted UC IVINS on with
escribed IVINS as a good u andjokester. I { Ias part of[§:E]initialbeb"/F
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FD-302 Rev. 10-6-95!
-1-
FEDERAL BUREAU OF INVESTIGATION
ALL II-IFDPHATEUI-I CIJIIITAIEJEI1
HEREIN IS U1*]i:L§1$SIFIEZI-
DATE 12-12-anus BY 50:24 UC EAHjDKfRT Dmeofuamcmmn 5 [0]_{2 !O6
On May 1, 2006,] ] ] date of birth 55
]Social Security Account Number SSAN!] ]was b7C
tele honically interviewed via work phone number of
]is currentlv employed] I
] lconfirmed that prior to working at[:::::::]was ii?employed as a] ]at the United States Army
Medical Research Institute of Infectious Diseases USAMRIID!, at
Fort Detrick, Marvland.] worked at USAMRIID from b6worked in the] b7c]Most of] research at USAMRIID was conducted in
Building] ]in the] ]suites, mostly[::]suites.[::] couldnot recall the exact room num er. I Ialso remembered sharing lab
space in]
also recalled working with a lab technician named
' |;| advise egg; I |BRt;cE92 IVINS g
indicated t at IVINS's
that Iso
b"/ T
] ]explained that] ]did not work directly with IVINS on
~ any research] ]
L [described TVTNE as ver Iivel ,]intelligent, and someone who likes to 'oke around a lot. f::::::E::L11did not work directly with
on any projects] ]
mwmgmmim O5/O1/2006 M Frederick, Maryland
File # 279A-WF222 93 6 USA1/IRIID " 92§SS ~ Date dictated
biby _ W
This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;
it and its contents are not to be distributed outside your agency.F/<é/I//'"'5- Zia? I
:1 N BG ~ ' §
FD-3 02a Rev. 10-6-95!
I
2'79AWF222936USA192/IRIID
Continuation of FD-302 of I I , On O 5 / O 1 / 2 O O 6 , Page 2
W ' ' with any emnJ_0w.ws cu" iers at
USAMRIID named did not
know former USAMRIID émployeeI I 92
confirmed that, while at USAMRIID, I:Idid some
research wor with Bacillus anthracis Ba! and be ' s it was the
Ames t bs rain ut was not one hundred ercent sure Iiffjconfirmed
workin with Ba spore preps alwa s obtained from ' ' _ I Y |:|
believed that o tained the Ba from a freezerIlocated in th "hot suites" and thatI I
advised that
has previously provided a more accurate and detailed account ofresearch work at USAMRIID to investigators.I Iadvised thar
once
Iadvised that this was the
general standard practice at USAMRIID.
[::::::]explained that did not conduct any research
utilizing a fermentor nor was involved in an research
pertaining to aerosol challenges or dry work. was not aware of
any dry work being conducted at USAMRIID. was unaware of any
plans in the future to conduct dry work at USAMRIID. [::]advised
that weaponizing Ba would be a big undertaking and a lot of effort.
[::]did not know how someone would go about doing that and[::]
would not know what type of equipment would be needed to aerosolize
Ba other than it would involve more than just a freeze dryer.
E:::::]did not use a lyophilizer while at USAMRIID but on a rare
occasion or two,[::]saw the lyophilizer being used for protein
studies.
[::::::]explained that while at USAMRIID,[:::]did on
occasion use an autoclave or place materials in an autoclave for
someone else to autoclave at the end of the day.[::::::]recalled
that there were two "Pasteur" autoclaves located in the hot suites
and that it would take approximately one hour to complete an
autoclave cycle.[:::]advised that when working with cultures[::::]
also would add bleach to the cultures prior to having the cultures
autoclaved. Employees could not leave cultures out in the suites
at the end of the day.[:::::]was not aware of any special or
additional security measures that were in place when working withIAmes Ba. I Iwas very picky about the quality of sporesr___Hb6
b7C
b6
b7C
b2
b7F
b/C
92 Y 1 ' R R é 0
FD-302a Rev. 10-6-95!
2'79AWF222936 USAMRIID
Continuation ofFD-302 of | | , on 0 5 / 0 1 /2 0 0 6 , Page _3__
L
maintained the Ba spores in a freezer. |:|explained that
petri ishes could be left out in the labs for a couple of days but
the dishes would be taped with para film. Streaked plates would be
kept in a refrigerator.
92
v
|b7C
i wn E
FD-302 Rev. 10-6-95!
- 1 _
FEDERAL BUREAU OF INVESTIGATION
ALL Il]FU]?!HATIElI~I CCIIJTAI1-J'E'.II' »
HEFEII1 I5 T_TI-IC_.ASSIFIEI1
],1g_'1];' 13- 1;;-;gl_1|_1:;: 1513* 5[_|:5;<:4 |_I|;f f;I§;_[|],>"J_11¢1_,»1~!_1"5 Date of transcription 5 Z 03 [2 0 Q 5
b6
20o6,I I 1:7cI Idate of birthI I Social SeduritAccount Number SSAN!I 1 YIwas
telephonically interviewed. This Inspector had left messa esearlier in the week at[:EEEEEEE3residence and on this date%:::::]
returned the phone call. was immediately informed that aninperson thorough interview needed to be conducted with[:£E}%ft
that some minimal preliminary information was desired from over
the telephone.
I Iconfirmed that is currentlyI I
indicated thatI Iis extremelbus and thatI__]is going to be X1 ' %' | ' I advised thatI will also be awa
I Iconfirmed that fromI
I Iat the United States Army Medical Research Institute of|Infectious Diseases USAMRIID!.I Iworked in the[::;EEEE;:::gbharacterize wor
responsibilities as thatiof a "worker bee".I Iadvised thatI[:]has
not been aI Iconfirmed that[::]did
work with Bacillus anthracis in the "hot suites" while at USAMRIID.
, [:::::]acknowledged knowing BRUCE§IVINSbut never workingdirectly with him.[::]indicated that on occa ionE::]would run into 1
IVINS while having a cup of coffee and remembere IVINS having agood sense of humor.I I
[:::::]was reinformed that a more thorough and indepth
face-toface interview with[:::]would be needed at a later date.
Investigation on O 5 / O 3 /2 O O 6 at Frederick , Maryland
File # 2 7 9AWF - 2 2 2 9 3 6 -USAMRI ID - 92§S92° Date dictated
b6by PI I I sic
This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;
it and its contents are not to be distributed outside your agency. _,</aw/15-11302 F
1'5' . 6;, ~ I1.
FD-302 Rev. 10-6-95!
-1-
FEDERAL BUREAU OF INVESTIGATION
ALL II~IF|II13!I{é1.TIIIlI~I CEIEITAII-JEII '
HEFEEIIJ I3 LT!-ICLASSIFIEIII
1.u=;1r 1.:-11>;-;::|.1|.1t= 1;: .5 r.~|;|:;-::2 4 LILY r;=m~1,-t1r:_.=.1~@.rs Date of transcription O 5 [1 3 [2 0 0 5
On May 9, 2006] Idate of birth
I I social security numberI I home telephone
number work telephone numberI I was
interviewed at place of employment, the United States Army
Medical Institute of Infectious Diseases USAMRIID!, BuildingI[:::]
E::::::::::] After being advised of the identity of the
interviewing agents and the purpose of the interview,[:::::]
provided the following information.
raduated with aI IDeqreeb6
b7C
I I
IAfter submitting]
assisted on a project involved with non-virulent Bacillus anthracis
I From this research,I I
, I Most of the work
conducted was on the of Bui ingI Iin Suitealso worked o%_EHET::£:::::]of Buildin in Suite
did not conduct research in Building and did not ave
access to that building. [::]stated the animal work was conducted
' B ' in where the pathologist would do necropsies, and
would work with the All Glass Impingers AGIS!.
has no experience in this type of work. The microbiology
work included plating and blood culture work, and would be done inBuldingj Irecalled doing copious amountsof
Ill
Investigation on O5/O9/2006 at FOIIZ D¬tI.iCk, MD
File # 2 7 9A-WF- 2 2 2 9 3 6 -USAMRI ID ' Date dictated
PI
by PI F
This document contains neither recommendations nor conclusions of the FBI. It is the property of I and is loargi to your agency;it and its contents are not to be distributed outside your agency. WPI I Iresides atI | IinI Ifrom the University Egéisl
92?-,9
b6b7C
b2
b7F
I3:
~; rs g E
FD~302a Rev. 10-6-95!
279AWF222 93 6USAMRI ID
Continuation of FD-302 of _ , On O 5 / O 9 / 2 O O 6 , Page 2
I Iattended the University of
, b6
b7C
I Istated security at USAMRIID is now tighter than
[:::::] knows BRUCEQ§%l§§ and the have worked in thehotsuite at the same time toge her. [:::::2j<described IVINS as astrange guy. E:;lelaborated that IVINS's quirkiness likel comesfrom a hard c l hood and not having many friends. [:::::%::]
impression is that IVINS "lacks social graces" and is ine t whenJinteractinq with people. I Ifurther stated| l I |
II lildoes not 1m<>w|:|and has never seen
Althou h the have never collaborated on any projects,
|knows| Isocially. Interactions included a
is not familiar with| |
does not recall working with any foreign
____,_
l L 9 ' P I $
FD-302a Rev. 10-6-95!
b6
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279A-WF-222936-USAMRIID
cmmwmmomnsmor I I ,OnO5/O9/2006 ,m@ _3_
scientists. [::::::]stated that none of[::] coworkers expressed any
antiAmerican sentiment, nor did they try to circumvent any
security procedures.
[::::::]is not aware of any dr rk having bcurrentl being conducted at USAMRIID. f:%Tstated thatTff:fj[:::::::::f::::]certain1 would not have been privy to such aconversation and that[%:]was only familiar with wet spore preps.The first timi£EF:::] heard of work with dry Bacillus anthracis was
in an article read about U,S. ARMY DU@WAY ROVI .GROUNQ fromthe media. When questioned about other laBs,i Imentioned thatit was rumored that §AIIELLElEQRPORATION messes up animal studies
and was not considered to do good work [:]knows_BI ORICORPOEQTION has Anthrax Vaccine Adsorbed AVA!, but[%¬Thas no
personal interaction with them and has not heard any negativecomments. [;;::::::]best theory pertaining to the anthrax mailingsof 2001 is t at a terrorist could have been involved but[::]has no
basis for that conclusion.
|
92 , .~"¢ s I 0- 9 ' O
FD;302 Rev. 10-6-95!
-1-
FEDERAL BUREAU OF INVESTIGATION
ALL I§~IFClP!I{ATIlIII~i' CIIIEEITAII-i'1ilIJ "
I"[EF-TEIZJ IF] UTIIELAASSIFIEII
DATE 12-12-ZUIIIFE BY $113211 UC BAI~I;'DEi_."RFS Dt a
__vte of transcription * O4 Z 2 5 [2 O O 6
On 24 April 2 006 , >7 Date, o_f_b_ir_th,I I I
I LSoc_f S_ecu.;:;;i..tv count, l92Ti1mber.. ,-_ girl ,_ _iinp J h e I I
wasinterviewed. The interview took pface atLt IE:::::::] .After being advised of the iden ities orinterviewing agents and the nature of the interview,%Ef::::::::]
provided the following information: '
Education and Work Experience
is currentlv emoloved b
wor ing as aI
I Iis currently assigned as theI I
InI Ireceived aI degree
rom Iwor ed atI I
started as
Iand held various oositions at thdb6
b7C
be»
b7C
culminating inI I IJ 92¬§§,
never worked with Bacillus anthracis Ba! at theI I
because the Lab was not equipped to handle Ba. Part ofthe researchI Iconducted at the I Iinyolygthe creation of aI
Investigation on O4 24 / 2 O O 6 at I I
usnmIz3:tb92S1,°3File# 279A-WF222936-E~P:BI I Date dictated O4/25/2006
SA b6
by sA_ ' b7C
This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;
it and its contents are not to be distributed outside your agency.Z
.;-..r-VSEARCHED EXED..___..
SERIALIZED D
» FB|||;921:|D92.@§; .w1*§92 U
BACK PAGE
-l> 2 . O " O
FD-302a Rev. 10-6-95!
279A-WF-222 93 6LEAD
cmmmmmonnsmor _4 I ;ono4/24/2005 Jae 2
In| lat the United States
Arm Medical Reggggghmgnstithte QfAInfectiousDiseases USAMRIIQQ,
iIl$:Q§ECMQ&~¥@;¥lQR@;T I
92
Work Environment and Colleagues
[:;:::::::]kws never dried or lyophilized Ba.
has used a ermenter extensively but never with Ba. as use afermenter with|_h knows
many people who ave knowledge of weaponization techniques, spore
production and lyophilizing. This list of people would be very
lar e as most everyone in the field of Microbiology thatE;:ii;:;::] knows or works with, would have some degree of such
now e ge. _ x
[::::%;:::]did describe one individual with weaponization
knowledge as a ittle stranqe. I I
'USAMRIID Questions
visited USAMRIID only one time for a
eriod in In c ' 'onnection w1th[::]research on the
conducted viability tests on live cultures of both themes an Vollum strains of Ba. [:::::::::]was escorted at all
times while at USAMRIID and was never left alone. [::::::::::]wassupervised by Bruce92§vins, but does not remember the names of any
of the other staff a USAMRIID. No one at USAMRIID struck -[;;;;;;;;;]as a possible perpetrator of the anthrax mailings. -
described Ivins as "a typical geek scientist whopro ably spends most of his time in the lab."i:::::::;::jsaid that
in the Microbiology community, such scientists are re erred to as
"SCIF dwellers."
has never received any biological samples from
USAMRIID. only recalls working in two labs at USAMRIID,
Idoes not
j I Yb6
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FD-302a Rev. 10-6-95!
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b 6Continuation of FD-302 of | , On O4 / 2 4 / 2 O O 6 , Page _3 by Q
recall ever entering or looking into a walkin cooler in either
lab.
informed that has a copy of a report
summarizing work at USAMRIID on computer and would supply
it to the FBI at a later date, once locates it.
Questionable Behavior
1 [:;;::::::]opined that the only way it would be possible
to remove se ect agents from a USAMRIID lab's hot area, would be to
» secret the agent inside your body. [:::::::::]added that this
would be difficult to do, as there are always at least two people
inside a hot area and the people would be in close proximity. A
person would not be able to hand carry a select agent out of a hot
area, because a person exiting must remove all clothing and go
through a wash down process. The only other means to exit the lab,
would subject any items being removed, to either an autoclave or an
ultra violate cleansing process. .
§;:::::;;;]does not know of anyone who exhibited any
behavior t at wou indicate that they would steal pathogens or '
toxins and use them inappropriately.
Travel
has never been to
Jersey, nor as een to Costa Rica.
' 1
Miscellaneous i
[::::::::::]does not know anyone who did an unofficial orunrecorded work with Ba or its simulants. [::::::::: has never
seen or used pre-stamped envelopes, like the ones utilized in the
Ba mailings.
does not have any knowledge of[::::::] and has
never met Trenton or Princeton, New
r92
1 1!
. ill . IF
FD-302 Rev. 10-6-95!
-1-
FEDERAL BUREAU OF INVESTIGATION
ALL II-IFUHFATIDI-I EDI-ITELIBIED
HEPEIIII IS UIICLAHHIFIEID
mart 12-1 -ants 5|:-2: 4 U: BAT.-I;"DE_.*"FCfS Date of tfanscpon Q 7 {O 5 [2 O 0 6
I hSocial Securit Account Number SSAN!:I I Date of Birth DOB!:I I was ifinterviewed, as pre-arranged, residence address:I I I
I I on the afternoon of June 28
2006. After being advised of the identity of the interviewing
Postal Inspector and Special Agent SA!, and the nature of the
interview,[::::::::::::]provided the following information:
was provided a NonDisclosure Agreement Form
for review. verbally agreed to items listed therein;
however, refused to sign. WFO NOTE: maintained in the FD34O
portion of this communication is the above mentioned NonDisclosure
Agreement Form[::::::::::::]verbally agreed to.!
I Iis ai I
At present,I I
advised[:::]had not recalled any additional
information since last interview circa 2003. WFO NOTE:was provided with a printout from
for review, which is maintained in t e FD-340 portion
o t is communication !
i I Iadvised[:::]could not recall.[::::::::]
I Iever callingI Iat home from[:::]United States Army
Medical Research Institute of Infectious Diseases USAMRIID! work
telephone on the afternoon ofI hffirmed
the information on the provided printout was accurate, and that
durinqI Iresided atI IL I
lhasI
I35
IQ/IA/-be
b7C
b7E
[IIIIIIIII;;l@pined as!andI Iwas apparent v at homeII IandI Iresidential telephone number #! wasI I
ié21qi@II opined
given the relative short duration of the call, and the
time frame,I Ima have calledI Ipertaining to a researchproject at USAMRIID. I Iwas knowledgeable of Z302
Hw# 279A-WF222936USAMRIID- Dmemmmd N/A
PostaIIby SA I I
This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;Investigation on O 6 / 2 8 /2 O Q 6
b6
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I Ii
-1 E I a
FD-302a Rev. 10-6-95!
2 79AWF-222 93 6USAl92/IRIID
b *5Continuation ofFD-302 of ' i , On O 6 / 2 8 /2 O O 6 , Page 2 b 7 ,3
Iopined thertelephone conversation mav have pertained to] [effort to
advisgd
it was not uncommon for coworkers at USAMRIID to contact home;
however, reiterated[::] could not recall ever
contacting telephonically.
[::::::::::::]advised revious statements pertaining
to the relationship between USAMRIID coworker,
were correct as did not have a ersonal 92relationship with Iadvised
Furthermore, at that time
| |at USAMRIID and| |kept political andsocial opinions to| | '
. - I l Iadvised as al ' I
interacted with
Iindicated, as in| |priorinterview,| Icould not recall the specific laboratories within .
USAMRIID building accessed, other than to add they were
laboratories.
I
onl interaction wit wasY
4
' |I II ~ Q . 0
FD-302a Rev. 10-6-95!
279A-WF222936USAMRI ID
4
Cb t
Continuation ofFD-302 of I I , On O 6 / 2 8 /2 O O 6 , Page 3 b7 C
could not recall any specific work relatedconversatiggg:%;;hE;;;%:::]however, reiterated the substance of
such conversations would be restricted to topics such as schedulin
a time toI I Although
recalled a conversation at USAMRIID withI Iin which
EHIcouId notlrecall any additional specifics opined is type ofbehavior bvI Iwas typical £iE[:::::::]EEi consistent withI[::]
I IadvisedI Inever inquired aboutI I
I As an aside,I Inoted1 Idid not I
[::::::::::::]advised recollection of[:::::::]
ever talkin to anyone from the Division at USAMRIID.[:::::::::::%]further advised it was no uncommon forE:::;;::::]
Division personnel to collaborate with individuals from e
Bacteriology Division, and even ifI IobservedI Italking to
someone from the Bacteriology Division,I Iwould not have thought
of it to be a strange or unusual occurrence.
advised as a IE::]was aware t at t e ivision suites contained the
Ames strain of Bacillus anthracis; however the exact location s!within their suites was not known. [::::::::::::]opined that if an
individual was involved in the anthrax related aerosol challenges,
then an individual would have the opportunity to learn the
location s of the Ames strain of Bacillus anthracis within the[::::::::i:i:]Division suites and/or the postchallenge material.
conductedI Iand reiteratedI Inever worked with the Ames
strain of illus anthracis. Prior toI Ide arture fromUSAMRIIDII Iworked with BRUCE IVINSEID I
applying I
to Bacillus anthracis vaccine
research. -
4
' 0l ._ |
FD-302a Rev. 10-6-95!
2 79AWF222 93 6 USZ-XMRIID
Continuation of FD-302 of I I , On O 6 / 2 8 / 2 O O 6 , Page 4.
r___[::::::::F:::]thought IVINS was strange, but not in a bad
wav. further described IVINS as a strange duck.
Ithought IVINS was supportive ofI Ieven thou hI I
had noth' derogatory to say aboutI I
recalledI Ib6
b7C
I Irecalled IVINS wanted1 gtoIrecalled that or
to go outside ofI
Iopined it was not the case of
demonstrating an intent to hinder an investigation,rather in| |n1uiE::]probably did not want to spend[:::]
time and effort with FBI or in other words, nonscientific, issues.
[::::::::::]was typical of the civilian scientific
community at USAMRIID. Military_scientists were generally aware of
and concerned with security issues, whereas, the civilian
scientists were not concerned with security issues, nor safety
issues. In particular,[::::::::::::]indicated civilian scientists,' are lax in how they report hazardous maferial I I
incidents.
I Ispoke fondly ofI Iformer USAMRIID co-
workerJ I' I Iconsidered| I and a source of
information pertaining to current events at USAMRIID.
[::::::::::::]recalled additional details ertaining toforeign visitors at USAMRIID; however,I Iwas not
familiar withI I
' x
I92- %
FD-302a Rev. 10-6-95!
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2'79AWF-222936USAMRIID bI'!_r4
Continuation ofFD-302 of 4I I . , On Q 6 / 2 8 /2 O O 6 , Page 5
I Irecalled the name of '
I IadvisedI Idid not know if thatvisit ever occurred; howeverI Iwould query[:::::1
[:::::::::::]recalled[::]vms the USAMRIID point of '
contact POC! for an individual identified on USAMRIID records as
1 I Iopined USAMRIID
records were referring to| I
dldid not have access to
the hot suites at USAMRIID, rather presente research ertainin toI Iin the SAMRIID conference room. I I
indicated would check further to see ifI could definitively
identify and/orI WFO NOTE:
maintained in the FD34O associated with this communication are
Internet com uter printouts, provided by[::::::::::::] pertaining
to| I ,
I Icould not recall the name of a foreign
visiting scientist fromI I who[::]had previously
reported had been hotocopying too much information while at
did not reco nize the name| that the I scienfisfI was
remembering did not have access to e 0 suites at USAMRIID."
I [:::::::::::]opined althoughI Ihad
collaborated with numerous foreign scientists, and was the USAMRIID
POC for numerous foreign visitors, it was unlikely that[:::]
indifference, or lack of cooperation, in assisting the FBI in
providing personal identifiers which would fully identify the same
was the result ofI I
I Irather, it was likely that such an attitude
was endemic to most civilian scientists at USAMRIID. [::::::::::::]
opined[:::::::::]had a personal and professional responsibility to
provide the FBI with all pertinent personal identifiers.b6
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FD-302a Rev. 10-6-95!
2 '7 9AWF222 93 6 USAMRI ID
b VContinuation ofFD-302 of il I , On O 6 / 2 8 /2 O O 6 , Page -6- b7
advisedlimmuwould ch rchived email
surrounding t e time frame of the telephone calltc>E:;]home residence and provide any results to the interviewing
Posta Inspector or SA.
Through investigation and interview[::::::::::::]is
further described as:
lI_NAME4
MlQQLE_NAME=
FIRST NAME:
SEX§
BASE:
SEAN:
, DQB:
HOME ADDRESS:
HOME TELEPHONE: "
"PE IOUS HOME ADDRESS:
LAST NAME:
E1Esm_NAME=
§§3
»#l: AFFILIATION
AFFILIATION #2:
-i
MISCELLANEOUS: ,EREVIOUS_HQME_IELEPH©NE<
Through investigation[::::] is further described as;
,_l_ _vi.
4r~ "J 92
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FD-302 Rev. I0-6-95!
-1_
FEDERAL BUREAU OF INVESTIGATION
ALL I]5IiFI3FLI~L'ITIE|1-I EIJIFTAIIGED
IIEIPEIH I15 UIIIIILASSIFIED Date of transcription O 8 Z 3 Q Z 2 O ! 6DATE l2~l2~2CIEIB Bit E~E-T32-4 TJC BAIII.-DI'L>P.f3
r__________1On August 28, 2006,| ldate of birthSocial Security Accoun umber[_ Icellular betelephone number] Iwas in I b7;
emplovmentj
Iwork telephone number] ' I
|After being advised of the
identity of the agents and the purpose of th e interview,[:::::::]
provided the following information:
[:::::::]confirmed that[::]was employed with the United
States Army Medical Research Institute of Infectious Diseases
_ USAMRI ID! froml I bél
307C
3%
I Idescribedl Ias sgmegne who isl I
b6
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Investigation on O 8 /2 8 /2 O O 6 at FOIt Detrick , MD
Filo # 279AWF222936USAMRIID 92§Q92b< Date dictated O8/30/2006
PIby SAI b 6
b7C
This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;
it and its contents are not to be distributed outside your agency.if/46/1»/W |:F3<1Q I
v
x m
FD-302a Rev. 10-6-95! Q
2 '79AWF222 93 6 USAl92/IRI ID
I3 FContinuation of FD-302 of I I , On O 8 / 2 8 / 2 O O 6 . Page 2 C
kII:::::::]advised that shortly after the above described
incidentI I Iexpounded Ehat it wasIUUUIbeI1efI Ithat career government employees at USAMRIID are
not as motivated to complete tasks and/or research projects in as
timely a fashion as noncareer contract employees for fear ofhaving the funding for their proiects eliminated.I Ilast
s oke toIp IOther than that,has not had any other communication w1thI I
I Iadvised that on several occasions
confirmed thatI Iknew whoI Iwas and whatI Ilookedlike. [::Fdvised during the interview and durin a brief followuptelevhone conversation, thaLI__lre2alled_seeinQIg______land
Iadvised that the onl other
USAMRIID employee that may have s ent more time withI Iduring his tenure at USAMRIID F I
explained thatI
I Iwas asked specifically to elaborate on[:;:]use ofI Iand its use regardin the growth of Ba. ex ainedthatI gIworked only with thestrain of Ba and that they grew the Ba inI
indicated that the inoculation period for the growth of Ba
,encompassed approximately eight hours and that one of
rimary work functions during the Ba related research was toIp Iexplained that Ba was harvested in a
FD-302a Rev. 10-6-95!
279AWF-222 93 6USAMRIID
Continuation of FD-302 of ' I , On O 8 / 2 8 / 2 O O 6 , Page 3
When asked about USAMRIID scientist _13RUCE92IVlNS, | |
responded that IVINS was a nice individual, but was not organized
and described him as "goofy." [::::::]recalled an incident involving
IVINS that took place several years ago at USAMRIID whereby IVINS
became frantic about the possibility that a shipment of Ba from
' g Grounds had been missent via Federal Express to the
offices in Washington, D.C. IVINS was extremely alarmed
an oca t roughout USAMRIID because he had heard on the news that
a package containing anthrax had been sent to in
Washington, D.C., and was concerned that this was he same
Ba that he was anticipating from Dugway. advised the
incident turned out to be a hoax mailing w 1C contained a petri
dish of a nonharmful substance. IVINS did receive his parcel of Ba
as anticipated without incident. [:::::::]indicated that because of
this incident and the hysteria created by IVINS, the hierarchy at
USAMRIID ultimately changed the way in which biological agents are
shipped to and from USAMRIID.
[::::::]insinuated that security at USAMRIID, particularly
prior to September ll, 2001, was extremely lax.| Idescribed an
inciden
questioned or wifhout Ehe coolers being searched.| |insinuated
that[::]was always amazed at the level of security that existed when
entering USAMRIID compared to the lack of security demonstrated when
leaving. "
advised that USAMRIID employee
worked a lot with and would possibly know etter
than most employees.b6
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n92K 92-
FD-302a Rev. 10-6-95!
2 7 9AWF222 93 6 USAMRI ID
Continuation of FD-302 of I I , On O 8 / 2 8 / 2 O O 6 , Page A__
As far as someone at USAMRIID having a possible motiveefor b*>
the anthrax letter mailinqs, I Iadvised at the only employee b73|:|could think of was| J uibased this beliefbecause of the nature in which] I
|:| .
I
LL I§~IFlIlP!l-IATIIIIDI CElZ~I'IAII-TEZI3 .rt = , _t;~ P-TEIZJ I5 UTJELASSIFIEII g I
FD-302 Rev,1 !-6-95! FATE l2l22UlI!F3 BY 5Cl32¢l UC 1':=_it.I*.T,>"DI&'_.I'13L F?
_1-
FEDERAL BUREAU OF INVESTIGATION
Date of transcription ! 9 Z O '7 Z 2 Q O 6
' I I date of birthI I social
security numberI _ {_ Lhome addreggj I
I __ I home telephone I Iwasinterviewed atI Iplace of employment, the United States Arm
Medical esgarch Institute of Infectious Diseases USAMRIIDIAB i RoomI 5IFort Detrick, Maryland, work phone number_"After' eing advisedlof the Identity of the
in erviewing agent and the nature of the interview,[::::::]provided
the following information:
InI Ibeqan emplovment at USAMRIID in the
I Ibegan workingin the hot suites after completin Iimmunizations approximately
I Iworked with lab
located in Buildind I The researc exclusively involved the
study ofI Recalledthat SuiteI Ihad
access to Suitd Ifor approXimatelyI Icould not recall the room number of Ilab in SuiteIdid recall that| |lab was locatedI L1
r r ll d hat a walk-in refrigerator
Petri plates, bottled
growth media and test tubes were stored in the walkin '
refrigerator. [:::::]stored bottled growth media there.
did not maintain cultures in the walk-in refrigerator. is
not aware of any material that was associated with Building
being stored in this walkin refrigerator. [::::::]was aw thatthe Ames strain of Bacillus anthracis is located in SuiteI IbutI Idoes not know the exact location. BRUCE IVINSI J
were responsible for distributin the Ames strain of Bacillus Ith ' 1; h |:g'| 11 h dldB '11 an racis o researc ers. persona y never an e aci us
anthracis. [::::::]recalled observin a fermentor through a windowin Suite Q that was assigned to The fermentor was to
be utilize to grow Escherichia co i for a arge scale protein
purification project. [::::::]is not certain if the fermentor wasactually placed into service. E:g::::]does not work with any
lyophilizers or freeze dryers an is uncertain as to their locationat USAMRIID. [;;:;;]does not recall noticing anyone that did not
belong inside t e ot suites. E:::::]has no knowledge of anyone
who might have been involved in the mailings of the anthrax tainted
letters. '
Investigation on O 9 /O '7/2 O O 6 at Frederick , Maryland
File # 2 '79AWF 222 93 6 -USAMRI ID -923°923 ' Date dictated 11/ a1:6
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1:6
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139
107?
bt»
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w I IPostal Inspector
" l
F » » O O
FD-302a Rev. 10-6-95!
279AWF222936USAMRIID
Continuation of FD-302 of _ , On O 9 / O '7 / 2 O O 6 , Page -2-
worked with|36
has land has W
in developing aerosolizaEionprojects
involving| Iis not aware of any research
involving the aerosolization of drv material. has since left
USAMRIID for a position at| left due to thepolitics and paperwork associated with USAMRIID.
has assumed
Anarimarily worxs at E:£]desk.Jis unsure o name, is responsible for
schedufing the aeroso ization studies. '
[::::::]has observed the following individuals working in
Suites B3 and B4 of Building 1425:
as leit US%éRIIDiQ@@ has eft SAMR ID!
currently conducts research in Suite[:::]of
Building has observed the following individuals in
this location:
I I» V
In Li- _ L
I
'7 92 Q1-IEREIIEI I5 UT-IELASSIFEEZI ~§LL INFORMATION CJNTAIHED 92 ~- *
*!!s Ow.0L3L2mB! Mg _ DATE 12-12-2005 BF amass UC BAwfDK!=
UFWEIJlEFlI92L.I3lJFlEJJ C!FIIlUWEEFT1 iAUTI !lI
Precedence: ROUTINE Date: O9/O6/2006
To: Washington Field
From: Washington Field Office
Amerithrax #3
Contact: SA | |
Approved By:
Drafted By:
Case ID #: g_79'A"-wCFE2A22§§_3.6;U_sZ§MRI1%} Pending! ~92E>°9292
Title: AMERITHRAX;
MAJOR CASE 184 _
Synopsis: To memorialize the work performed on the electronic
evidence collected on Bruce Edwards Ivins in relation to Major
Case 184.
Enclosure s!: One CD containing all files that were
"bookmarked" during the review process.
Details: During the course of the Amerithrax investigation,
Bruce Edwards Ivins consented to having his home computer, two
United States Army Medical Research Institute of Infectious
Diseases USAMRIID! workstations, various loose media items
and his USAMRIID user directory copied for review by
Amerithrax investigators.
The abovementioned items were imaged by Washington
Field Computer Analysis Response Team CART! members. The
imaged drives were given 1B numbers and were maintained as
evidentiary items. The imaged drives were loaded onto a
workstation maintained in the Amerithrax workspace located in
the Northern Virginia Resident Agency. Once loaded onto the
workstation, the images were loaded into the software program
I lscanned the imaged drives and
categorized each file based on its type i.e. email, document,
spreadsheet, database, etc.!. The files were then reviewed by
the writer of this communication. Files that were deemed
pertinent to the Amerithrax investigation were "bookmarked."
Writer then exported all files that were bookmarked to a CD
for review by other Amerithrax investigators.
Q?Z§fC!£!.bIPc! [::;1L02
io,-"Eb6
bi-"C
106
b"-C
b6
b'7C
92 ,92 I I
1.To: Washington Fgd From: Washington Fiel?Office
Re: 279AWF222936USAMRIID, 09/06/2006
Each image reviewed had numerous bookmarks that
separated each pertinent file into a specific case related
category i.e. Ames, Anthrax, Bioport, etc.!. Below is a
listing of each bookmark category, a brief description of each
category and the number of files bookmarked for each image:
Ivins' Home Computer0 b6
represents files written by or written about Ivins' b7C
| Four files bookmarked.
represents files written by or written about Ivins
| Two files bookmarked.
Anthrax files discussing anthrax in a general sense. 61
files bookmarked.
Blindfolded files associated with blindfolding, kidnapping
or other similar interests. 147 files bookmarked but numerous
files were not bookmarked because they were repetitive in
nature.
Contacts individuals listed in Ivins' "Contact List." Seven
files bookmarked.
Followup files that represent items that need to be
investigated further. 2 files bookmarked.
¬;:;:;;] items written by or written about[::::::::::::::::]
1 es bookmarked.
Follow Up Items ~ Same as Followup listed above. 38 files
bookmarked.
Google Searches Google searches performed by Ivins of a
curious nature. Need to be investigated further. 19 files
1 bookmarked.
Health - file where Ivins discusses his health. One file
bookmarked.
[::::] file discussing[:::::::::1 One file bookmarked.
KKG files associated with the Kappa Kappa Gamma KKG!
sorority. Nine files bookmarked.
Kingbadger file about Ivins' screen name kingbadger. One
file bookmarked.
2
To: Washington Fgld From: -Washington Fiel Office
Re: 279AWF222936USAMRIID, 09/06/2006
lit files dis@usSing|:| Two files
ookmarked.
Phoenix file where Ivins posts to an internet bulletin board
about fraternities/sororities using the screen name Phoenix.
One file bookmarked.
Abortion files mentioning abortion. Two files bookmarked.
Docsnivi54 file where Ivins uses the screen name docsnivi54.
One file bookmarked.
Loose Media Recovered in Ivins USAMRIID Office
3 plates file where three plates are shown in a photograph.
One file bookmarked.
Accomplishments files where Ivins] I
discuss their scientific accomplishments in relationship to
USAMRIID. These appear to be done on an annual basis. 15
files bookmarked.
Amerithrax - files associated with the Amerithrax
investigation. Two files bookmarked.
Ames ~ files mentioning or discussing the Ames strain of
Bacillus anthracis Ba!. Six files bookmarked.
Anthrax - files discussing anthrax in a general sense. 18
files bookmarked.
Bioport files discussing Bioport. Two files bookmarked.
Bruce's Swabbing - file discussing Ivins unauthorized
environmental swabbing at USAMRIID. One file bookmarked.
Dugway files discussing Dugway in general or the contract
where USAMRIID paid Dugway to produce Ames spores. 79 files
bookmarked. .
FVS short for Foreign Visiting Scientists, these are files
discussing foreign scientists at USAMRIID. Two files
bookmarked.
Follow Up Items Same as Followup listed above. 28 files
bookmarked.
[:::::::]- files discussing| I Seven files
bookmarked.
3
1
J
I
1
14
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a
92
I
i
92
92
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Re: 279AWF222936USAMRIID, 09/06/2006
Papers represents published papers written by Ivins. 19
files bookmarked.
Patent files discussing issues surrounding Ivins' patents.
Two files bookmarked.
Procedures - represents Standard Operating Procedures, or
protocols, used at USAMRIID in experiments. 16 files
bookmarked.
Recipes written recipes used to grow bacillus spores. Eight
files bookmarked.
Ivins' USAMRIID User Directory
1029 files referencing RMR 1029. Eight files bookmarked.
1412 files referencing Building 1412 at USAMRIID, Fort
Detrick, Maryland.
AMI - short for American Media Incorporated. Three files
bookmarked.
Abortion files mentioning abortion. Two files bookmarked.
Accomplishments file where Ivins discussed his scientific
accomplishments in relationship to USAMRIID. These appear to
be done on an annual basis. One file bookmarked.
Address Book files from Ivins' address book. Two files
bookmarked.
Ames files mentioning or discussing the Ames strain of Ba.
97 files bookmarked.
Anthrax Conference in 2001, USAMRIID sponsored an Anthrax
conference that was held in Annapolis, Maryland. Files
discuss this conference. Two files bookmarked.
Battelle files that reference Battelle Corporation. Two kg
files bookmarked. °b7C
newspaper article discussing the search of[:::::::]
homes. One file bookmarked.
Bioport files discussing BioPort Corporation. Four files
bookmarked.
4
To: Washington Fld From: . Washington Fie]gOffice
Re: 279AWF222936USAMRIID, 09/O6/2006
Kappa files associated with the Kappa Kappa Gamma KKG!
sorority. Three files bookmarked.
files discussin I Iw I1 files bookmarked.
files discussinglm I
Three files boo arked.
[:::::::]Review Items bookmarked th cientific innature to be reviewed by SA[::::::::ff:%fff:fAmerithrax #2.
75 files bookmarked.
E;:;;;:;;- files discussing[::::::::::::::::] Three files
oo ar ed.
Mailing Reference files that discuss the anthrax mailings.
Two files bookmarked.
Mikesell Files referencing Perry Mikesell,
scientists at Battelle who is now deceased.
bookmarked.a former
One file
Misc. - miscellaneous items pertinent to the
investigation. 21 files bookmarked.Amerithrax
Patent ~ files discussing issues surrounding Ivins' patents.
32 files bookmarked.
Pictures Anthrax pictures of anthrax. 24 files
I bookmarked.
I
I Potency Team files associated with Ivins' membership on the
anthrax vaccine potency team, 33 files bookmarked.
SOPs represents Standard Operating Procedures, or protocols
used at USAMRIID in experiments. 27 files bookmarked.
Timeline files to be used to timeline Ivins' whereabouts
during the mailing window. Four files bookmarked.
Vollum 1B 189 files discussing Vollum lB 189. Seven files
bookmarked.
6
i files discussing] I Eic
I Four files
ookmarked.be
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To: Washington Field From: Washington Field Office
Re: 279AWF222936USAMRIID, O9/O6/2006
[:::::] files referencing|
Two files bookmarked.
FBI files mentioning the FBI or employees of the FBI. 37
files bookmarked.
Ivins' Office Workstation
Ames - files mentioning or discussing the Ames strain of Ba.
Four files bookmarked.
Anthrax files discussing anthrax in a general sense. Six
files bookmarked.
B3 files discussing USAMRIID laboratory B3 within Building
1425.
Blindfold file discussing blindfolding. One file
bookmarked.
Bruce's Swabbing file discussing Ivins' unauthorized
environmental swabbing
Contacts individuals
files bookmarked.
FBI - files mentioning
files bookmarked.
Follow Up files that
investigated further.at USAMRIID. Two files bookmarked.
listed in Ivins' "Contact List." 23
the FBI or employees of the FBI. 22
represent items that need to be
45 files bookmarked.
|:|- picture of
One file bookmarked.
Lyopholize files referencing lyopholization. Two files
bookmarked.
[:::] files discussing[::::::::::::::::] One file bookmarked
Misc miscellaneous items pertinent to the Amerithrax
investigation. 2 files bookmarked.
SOPs represents Standard Operating Procedures, or protocols
used at USAMRIID in experiments. One file bookmarked.
Kappa files associated with the Kappa Kappa Gamma KKG!
sorority. One file bookmarked.
7
To: Washington F£Ld From: Washington Fiel?Office
Re: 279AWF222936-USAMRIID, 09/06/2006
B5 Workstation used by several individuals including Ivins
Follow Up file that represents items that need to be
investigated further. One file bookmarked.
Summary
Each of the abovementioned bookmarks and files can be found in Fa
the [::]generated report located in the enclosed CD. g§C
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Precedence: ROUTINE Date: O9/O7/2006
To: Washington Field milmmmnmnmummunmt
HERETM TH nmtnissTFItn
From; washington Field mm 12-12-zmua 50224 UC EllIII;"DK_.*';ff:":1
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Case ID #= 279AWF222936USAMRIID Pcnding!~92E§lQ5
Title: AMERITHRAX
Major Case 184
Synopsis: To provide a periodic update regarding identification
and tracking of fermentors in place at the United States ArmyMedical Institute of Infectious Diseases USAMRIID! prior to [iii]
the anthrax mailings, obtained from collected documentation and
reviews of FD-302's.
.r;H Details: One fermentor was located and posit ' ' ' ashaving been in place at USAMRIID, in Building1fiii:ifffiifif?]
around the time of the anthrax mailings. Additional fermentors
have been identified with an undetermined final disposition date.
Available Propertv Accounting Online Maintenance Records were
collected froml lat USAMRIID, Q6
and| J_ I Iat USAMRIID. blc
Maintenancerecord information includes the hand receipt holder
code, the stock and model number, and various service dates. The
equipment is also identified by an internal USAMRIID code called
the MMCN number.
l50Liter Fermentorz R0om[::::]
The TF15O New Brunswick fermentor, MMCN Number b5
Stock Number] Iwas known as_§iq3Bertha. b7@
I I Throughdut variousb2
b7F interviews this large or bulky fermentor has been described with
volumes varying from 100-Liters to 300-Liters by the interviewees
best recollection. It was installed into[::::]after the removal
of the wall because it was too large to fit through the doorway.
The fermentor was not portable. recalled that there
were two of these fermentors in Building The were bothacquired between 1980 and 1985.2 .As arrange y[::ft::] the
b6
b7C
[;;]@q¢6.mP!J
, ,To: Washington Fld From: Washington Fie
Re: 279A-WF-222936-USAMRIID, O9/O7/2006
¢@¬2a¢t basis, supports the research of laboratories by
providing exper ise in cell culture, protein expression, and
protein purification as well as growing cells. The contract
describes the relationship between the two entities as,
"Temporary housing and maintenance of USAMRIID fermentors in
exchange for unfettered use by a contractor lus scheduled runsfor USAMRIID."3 A.copy of a photograph fromE:::::::]IEAMRIIDHorizon newspaper article shows Big Bertha and the two,Bi§§taE
_Braun Biotech92fermentors at their current offbase location.fermentor located in IL I i _ I On a
[:::::::::::::] stated the fermentor was used fairly
infrequently due to its larqe size. It has had various
operators to include:| | material
used C. Botulinum between 1991-1993; material usedE.Coli B. Subtilis, and delta-Sterne betwee 1991-1997;6E:::][::::%:j material used FV1 antigen-I ! |materia usedSter e strain of Ba;| | material used water.
During |employment individualsrecalled that used thé fermentor in RoomE:::]for attenuated
strains of anthrax; protective antigen PA!, lethal factor LF!,
and edema factor EF! studies. | |
and has fermented B. subtilis.
[:::::] described a second fermentor as being Big
Bertha's twin. It was housed in the B4 suites and was
d to and |Eifiifffffi It is surmised that after the destruction of the GMP
facility at USAMRIID this fermentor was relocated and therefore
not in place during the anthrax mailings.
[:::]who was identified as using Big Bertha and its
twin, originally obtained these fermentors from the Salk
Institute for use with C. botulinum in direct support of vaccine
production with a deadline from the Pentagon in support of
Operation Desert Storm. Around that time frame, the fermentorswere both located at USAMRIID in Building[::::]in Biological
Production Lab 1 [:::] and now known as rooms andrespectively.8 [:::] [::] [::::]
75Liter Fermentor: Room[:::::]
This fermentor, no MMCN Number, no Stock Number,
described onlv as beinq qlass-lined was housed in[::::] during the
1980's. I Ia 75-liter fermentorlocated in Room| |of Suite] lto grow colonies of attenuated
delta-Sterne strain Bacillus ant racis."9 Other operators
2
1b6
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b2
b7F
,To: Washington Fgld From: Washington Fiei
Re: 279AWF222936USAMRIID, O9/O7/2006
include:[:::g::] Big Bertha replaced the older 75Liter
fermentor, t erefore it is surmised that the 75Liter fermentor
would not have been in place during the time of the anthrax »
mailings. Final disposition records for this fermentor were
unavailable. _
20Liter Fermentor:[::::]
The Biostat Braun Biotech fermen
Stock Number 66400lCO60025 was located in fa?T Operators included and Q2I I This fermentor was relocated to KBI in 2005 375
30-Liter Fermentor:[::::]
The Biostat Braun Biotech fermentor, MMCN NumberI IStock Number 664O0lCO6O026LIwas located inI I' l I No ator in orma ion avai a e.This fermentor was relocated to[ffijin 2005.
20-Liter Fermentor: Room[::::]
The New Brunswick fermentor, no MMCN Number, no Stock
d in[::::] Operators included:I landrecalled that, in 2000,LIb2run a New Brunswick 20liter fermentor which
study pertaining to the growth of delta-Ames
Final disposition records for this fermentor
5-Liter & 2OLiter: Fermentor
This fermentor, no MMCN Number, no
d bl l t dl Iinvolved a vaccine
or deltaSterne."B
were unavailable.
Stock Number wasused for pilot runs an was possi v oca e in [:::;;:;;;]
Operators included:I Imaterial use r for
3anthrax vaccine in the 1990's. "In the 199Os,[:;::;::::::::]I I under t e irection of
were tasked to produce a recombinant version of PA rPA!..."
Final disposition records for these fermentors were unavailableHb6
b7C
/-
b6[:;;;;]indicated that the newer fermentors were the b7c
Braun Biotec ermentors and that the New Brunswick fermentors
likely predate 2001.
b6
b7C
b"/F
,To: Washington Fld From: Washington Fie,
Re: 279AWF222936USAMRIID, O9/07/2006
5 Liter Fermentor: Room[::::]
The Bio F nswick fermentor MMCN Number[:::::::]Stock Number was located in[:::]. ii?
b6 Property Accounting ' n n R
date 05067 ' the
included: material used de1taSterne.lent tol iby Bruce92Ivins.H It was used
fermentation runs. It is possible that the
mentioned above used for pilot fermentation runs and this[fifmfntor are one and the same. This fermentor was relocated to
1rd run
Operatb7c
This fermento®Z as
for pilot b7F
5-Liter fermentor
FBI Agent[:::;;:::%;:]vms contacted about the theory
of a feasible working vo ume or a fermentation run. He stated
the minimum volume would have to come from the manual of
operations for a particular fermentor but theorized that"a
fermentor would have to be at least 75% full to operate as
designed. In a 25OL fermentor this would he approximately
l88L. The culture used to innoculate this would be 19L, if
using 10% inoculum.
With regard to making a determination to examine the
fermentors more closely via environmental sampling the writer
believes that the l5OL fermentor "Big Bertha" would have been
difficult to operate innocuously and discreetl due to its largesize. The fermentors that were relocated toE:fj to include Big
Bertha! were probably deconned before leaving USAMRIID, although
no official documentation could be retrieved. At this time, no
official record of Ba Ames having been fermented was located and
the usage time frames, materials used, and protocols for the
smaller fermentors have not been fully determined. Additional
review of documents from92the Automated Logistics Requisitioning
s stem ALORES! from] |Eft:::]at USAMRIID is being compiled and reviewed for
identification information for the above mentioned fermentors.
Attached to and considered part of this documents is
the B3/B4 Fermentor Chart. .
The investigation is continuing.
4
|b
b7C6
_ ,To: Washington Fad From: Washington Fie
Re: 279AWF222936USAMRIID, O9/O7/2006
oo
1. ! FD-302 of| l sub USAMRIID Serial 1544
2. ! FD-302 011 Sub USAMRIID Serial 1532
3 ! Collected item 1A 6787 v O5.xlsJ:|Annual Inventory Check and
Correction, Property Accounting System Sub Hand Recei t, Solicitationl Contractl Order forCommercial Items and Statement of Work from
92 4. ! FD-302 011 Sub USAMRIID Serial 1544
5. ! FD-302 o - Sub P01 Serial 4330
6 ! FD-302 011 Sub P01 Serial 1501
7 ! FD-302 0f| Sub USAMRIID S6rial|:|
8 ! 0sA1v1R111> HO1iZO11 Article| I
9 ! FD-302 011 Sub P01 Serial 1501 I
lO. U! Collected item 1A 6787 |:lInvento1"y Nov 05.xls,|:|A11nua1 Inventory Check and
Correction, Property Accounting System Sub Hand Recei t, So1icitation/Contract/ Order forCommercial Items and Statement of Work o
ll. U! Collected item 1A 6787 |:|Inventory Nov 05.xls|:|A.nnual Inventory Check and
Correction, Property Accounting System Sub Hand Recei t Solicitation/Contract/ Order forCommercial Items and Statement of Work from
12 ! PD-302 Of| Sub USAMRIID Serial 1552
13 ! FD~302 011 Sub USAMRIID Serial 1532
14. ! USAMRIID Horizon Article | '
15. ! PD~302 01] sub P01 Serial 1501
5b6
b7C
$¢II _* W
ALL IEFIDRILETICIFI C[t11TEtI11"EI;t i
I[E1?LEI1E I3 T_11-]tILft5$iIFIED
DATE l2-iii-£3108 BY Ellifiiié LIZ EA11T,r"DlifP.f3
Fermentors
MMCN Common Name _ Typer7" . VolumeApproximate Stock Prev1ousW' ' Current Purchaser
ocation Location Date Operate?
0129.. 11- et'1..H@t1a'"'."'.' 92 TF¢1§0 _I1lgW Brynst111'_c! _ 150-1. 1 8
1 1980 1985
003 19801985
t 980 19
' 980-
IZ 2.ei1.e@1t@'=wnT"'
150-L 1980-1905__ 003 19801985
igiostatraun Biotech 20-L nla1nullIF4368 3. Femrentor
F4369 4. Femrentor Biostatraun Biotech 30-L nla
D5651 5. Fennentnf Bio Flow 111 NewBrunswick 3-L
. WV 19te§e:t11.e1!,.,., . 714 1/2-....... ' we
nla
All enlls with a slash mark
2. U! FD-392 n
3. U! USAMRIID Horbon Artist
4. U! FD~302 0
5. U! FD-302 01
R0v159<1 09/8106
J6.Fermentor
.. 1... Z-1'.e!9.?"!°1 .. .
mi Jun l
8.Fermentor r T _ 5-U20-L
Po Hraun Btotbch tnmtentors are newer and New Brunswick brand termentors probably predated 2901.
omtatlon! have no suppurtlng statements, documents, ut Indicators at thls tlme.
Sub USAMRIID Serial 1592
SuSub usmtnttn Serialm 135
_ e. U! FD-9020 Su
7. U! FD-302 0 Su
8.10! Fropeny ceeunltng ysem u nttReeelpt95067Approximate35'°"
Ttmetrame Used Material Used
Water
1991-1997 E.co1r', B.Sub1'11us, Delta-Steme
1992-1996 ___ Bacil1usan11§rct's PA!
,/ FV-1 antigen
I-Z StemeStrain
1991-1993 C.Botutr'num
1991 199_3_ Pen1avatentBotut1num Toxrod
1991 1993 Pentavatentotutmum Toxrod
994
2090 delta-Stemeordetta-Ames
Q 4-Q
1. U! Collected item 1A 67 nventory Nov05.xlsD92nnua| Inventory Check and Correction, Property Accounting System Sub Hand Receipt, Sotit:itatbn10nntrar:tl0rtter fcrcnmmercial Items and Statement of Wont Irena
11:6
1:713
' i i 0
FD-302 Rev. 10-6-95! i
-1-
FEDERAL BUREAU OF INVESTIGATION
ALL IEFDRHATIUH EUETAINED __I-[IiI1~'!J.'. it-J 1_1iii;ri.i:.s 5 J.I*'lEL1 , D"° °f "°"P° __L_LiO 9 1 5 2 O O 5
DATE 12-12-anus BY amaze UC B&HfDRjRTS
be
On Se tember 5, 2006, date b7¢
of birthI I Socialiegurity Ac unt Nu erwas interviewed atI Iwas recently I
IAfter being
advised of the identity of the agents and the purpose of the
interview,[:::::]provided the following information:
I Iadvised thatI I I
FIto the United
States Army Medical Research Institute of Infectious Diseases » USAMRIID! at Fort Detrick, Maryland. .
TheI Iat USAMRIIDII Iwork'ed as a 2
Ifirst worked forI II Iassisted in Iresearch pertaining to
E:::::]assisted with theI Ialso
assisted with theI Iadvised that.L_Jwas immunized against all a entsupon arriva to USAMRIID. ing this time period[:;::%:]neverwent into a hot suite, nor did[?fiwork with Bacillus ant racis
Ba!.I Ialso recalled working withI I
did not recall working with any foreign scientist;
however, does recall seein two scientists at USAMRIID whom b6believed were from known only toI:Ias bgc
b7D
Investigation on 0 9/ 0 5 /2 0 0 6 at FOIIZ Det1iiCk , MD
m# 279AWF-222936-USAMRIID~E3K§ Dmemmmd n/a
I Iby SA
_ b 6
. . . . . b7@This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency;
it and its contents are not to be distributed outside your agency. _F/4150/W4. |:|.&
|:|
i QFD-302a Rev. 10-6-95!
279AWF222936-USAMRI ID
13%
Continuation of FD-302 of I I , On O 9 / 1 S / 2 O O 6 , Page 2 4
and reiterated that duringIh IatUSAMRIID did not have access to any of t e hot suites.
Beginning on or around[:::] for approximately£::::::::][::::::::::::::::]advised that USAMRIID underwent a signi icant re-
organization resulting inI Ibein transferred to a new divisioncalled the! lnJ 31 This division was later I
only on the cold side of Buildinand recalled specificall workingonI _IDurin this time period,I IwokedI
I I conducted research pertaining to
research was done in lab roomsI I
advised that during this time period or
thereabouts, the Ireplaced the
I Ibegan working for inBuiIdlngI I lter viewing a floor plan of BuiId1n§I Iacknowledged that[:;}p£iTarily worked in roomsIadvised that most o work involvedI
Iresearch pertaining to andIOn a regular basis&::::::]aE§ly£edIand recalled on one or two occasions
conductingI in the hot side of BuildingI::::]
I Ialso recalled working with in the hot side
of Building[::::]for approximately
WFO NOTE: maintained in the FD-340 associated with this bf
'cation are USAMRIID floor plans which were provided to b°Effffjfor review.!
In ongoing attem ts to chronologically detail work
assignments at USAMRIID,I Iadvised that around
began working for Iin Building w otatthat time was the Iassisted with the b6
aerosolizin f the h ll t ' l d th ' ' l g o c a enge ma eria an e ensuing anima b7Q
challenges. I::]worked with many biological agents, including Ba.
also did some work for pr. BRU IVINS and recalled that the
Iworking for back then wereIdescribedI Irole with the|animal challenges as that of1 rl
[::::::]was asked to expound on the nature of[:::]
interaction with Ba during[:::]work at USAMRIID. [:]advised that1:771?
' ' O I
' FD-302% Rev. 10-6-95! ~
279A-WF222936USAMRIID b5
]:»7C
Continuation of FD-302 of I I , On O 9 / 1 5 / 2 O O 6 , Page 3
but was not certain if] Idid or not.| Irecalled havinq | |
to]
1 not know where the
or where at USAMRIID Ba was stored but recalled thatE:::::::::]brought the material over from Building 14;:i&???;;;;i?]
advised that part of| Iwork responsibilities included the
[::::::]advised that employees from other sections of
USAMRIID would often come over to observe the animal challenges.
[::]reiterated that there were a lot of people in and out of
Building[::::].After viewing the floor plans of BuildingE:::::]identified room[:::]as the lab Egjworked in. Room was
the redominant room used for aerosol c allenges. reiteratedthat%::]did not know where the Ba was stored and was never
responsible for retrieving the Ba material used for the animalchallenges.[::]indicated that[::]would not know how to grow Ba or
any other biological agents.- -
E:::::]was advised that a review of USAMRIID key card
access records pertainin to buildings[::::]and[:::]indicated thaton| qlhad accessed various locations within
both buildings at unusual and awkward times of the evening and
early morning. When asked to recall and attempt to explain the
irregular work hours on this date,[::::::]struggled to provide an
answer. Initially] |could not explain wh would have been in
the buildings between| or what type of
work or project| |may have been working on. After considerable,
thought and prodding,[::::::]opined that during the time period
immediately surrounding the terrorists attacks on September 11,
2001, USAMRIID officials, in response to the terrorists attacks in
New York and Washington, D.C., and rumors associating the anthrax|laced letter mailings with USAMRIID,| I |
The initiative was called the]
I I
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Icould not justify inI Iownmind what would have causedI Ito work the unusually awkward hours
onI Ialso intimated that around the time
period ofI Ihad been extremely busy and
working many late nights trying to finish a paper so that it could
be published as well as having to attend college classes in the
evenings. [::]did not recall having any involvement with research
in the hot suites of the Bacteriology Division during the above
mentioned time period.
In continued attempts to account for[::]presence in
Buildings 1425 and 1412 at th t ' e imes and date mentioned above,ex lained that[::]had no reason for being in any[::::::::;;
suites and that could only recall having accesse
a suite maybe one time in indicated that
most of[::]time at USAMRIID was spent in suites.
I Iadvised that at some point inI I
[:::::]was not aware of any dry work being conducted at
USAMRIID regarding Ba or an other biological agents.[::]advisedthatI Iwas involved in aerosolizing agents for
USAMRIID. advised that[::]had attended some meetings at
USAMRIID whereby discussions took place pertaining to a dry
aerosolizer but thatI Iwas not aware of an actual dry work being
conducted also recalled .E:] I Italking about dryingand/or aerosolizing materia ut t at it was just talk and that the
talk eventually subsided. This subject matter was discussed for ab¬
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0 tax 6FD-302'a Rev. 10-6-95!
279AWF-22293 6-USAMRI ID
cmmmmmonmamof I I ,OnO9/15/2006 ,Pge .5-
short period of time at several of the| I
meetings. [::::::]was not aware of the existence of any plans which
involved drying or transforming liquid Ba into a dry powder form.
[::::::::]opinions of the following co-workers are as
follows: _ _
1! BRUCE IVINS: Did not know very well, but thought he was very
serious about his work and thought he walked with a slight limp.
I2 |i'
3! I
4>l IIll
5>| I
I I
6U I
I I
7! [::::::::::::::] Did not know or offer any opinion of [::::::]
In closing,[:::::]advised that[::]never removed anyselect agents from hot suites while at USAMRIID.[::::::]provided
interviewing agents with[:::]bank account and visa credit cardaccount information in attempts to further account for[:::]
whereabouts during the time period of the anthrax mailings.was asked to submit to a polygraph examination on this date and
agreed to do so.b6
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