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Principal Investigator: Daniel Lingwood
Organization: HARVARD UNIVERSITY
Fiscal Year: 2024
Award: $411,028
Funding agency: National Institute of Allergy and Infectious Diseases
PROJECT SUMMARY/ABSTRACT: Advanced Lab Technologies Core
One of the greatest added values of the HU CFAR has been facilitating member access to new and emerging technologies. Science is accelerated by changes in technology, and the evolution of flow cytometric, imaging and genomic techniques continues at a rapid pace. However, access to such technologies and associated computational methodologies is an ever increasing and challenging barrier to individual investigators. Acquisition and optimization of instrumentation and software, and making them accessible, remains the primary focus of the HU CFAR Advanced Lab Technologies Core (ALTC). Making these cutting-edge technologies available to HU CFAR users adds critically needed value to their studies of HIV reservoir and cure, virus-host interactions, therapeutic interventions, and vaccine development. The consolidated ALTC offers a unified pipeline of immunology services, molecular biology services, and training opportunities strategically placed to serve the greater Harvard community at the most effective fiscal value. Indeed, the ALTC serves hundreds of HU CFAR users and their global collaborators supported by more than 100 NIH- funded grants/contracts across ten different NIH Institutes and Centers. The ALTC also continues to focus on providing critical individualized technical support to Early Career Investigators (ECIs), including 20 Developmental and Mentoring Core awardees. At the current time, much of the identified need for members is in the capture of information regarding the phenotypic and functional state of individual cells, indexing these samples, and then capitalizing on this precious biomaterial to develop a comprehensive picture of the genomic landscape that exists in particular disease states.
Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><AIDS><AIDS Virus><AIDS prevention><Acceleration><Acquired Immune Deficiency><Acquired Immune Deficiency Syndrome><Acquired Immune Deficiency Syndrome Virus><Acquired Immunodeficiency Syndrome><Acquired Immunodeficiency Syndrome Virus><Area><BS-seq><Basic Research><Basic Science><Bio-Informatics><Biocompatible Materials><Bioinformatics><Biomaterials><Biometrics><Biometry><Biostatistics><Bisulfite-based sequencing><Body Tissues><Boston><COVID-19 virus><COVID19 virus><CRISPR><CRISPR/Cas system><Cell Body><Cell Culture Techniques><Cell Isolation><Cell Segregation><Cell Separation><Cell Separation Technology><Cells><ChIP Sequencing><ChIP-seq><ChIPseq><Children's Hospital><Clinical Sciences><Clustered Regularly Interspaced Short Palindromic Repeats><CoV-2><CoV2><Collaborations><Color><Communities><Computer software><Computing Methodologies><Confocal Microscopy><Containment><Contracting Opportunities><Contracts><DF/HCC><DNA Molecular Biology><Dana-Farber Cancer Institute><Data Set><Development><Disease><Disorder><Doctor of Philosophy><Emergent Technologies><Emerging Technologies><Epidemic><Epigenetic><Epigenetic Change><Epigenetic Mechanism><Epigenetic Process><Evaluation><Evolution><Flow Cytofluorometries><Flow Cytofluorometry><Flow Cytometry><Flow Microfluorimetry><Flow Microfluorometry><Fluorescence><Fostering><Funding><General Hospitals><Genetic Alteration><Genetic Change><Genetic defect><Genomics><Geographic Distribution><Goals><Grant><HIV><HIV Infections><HIV Prevention><HIV/AIDS prevention><HIV/Mtb><HIV/TB><HIV/mycobacterium tuberculosis><HIV/tuberculosis><HTLV-III Infections><HTLV-III-LAV Infections><Health><Hospitals><Human><Human Immunodeficiency Viruses><Human T-Lymphotropic Virus Type III Infections><Image><Image Analyses><Image Analysis><Imaging technology><Immune Surveillance><Immunologic Surveillance><Immunologic Surveillances><Immunological Surveillance><Immunological Surveillances><Immunology><Immunosurveillance><Individual><Infrastructure><Institution><International><Investigators><Israel><LAV-HTLV-III><Laboratories><Lymphadenopathy-Associated Virus><M. tuberculosis/HIV><Machine Learning><Massachusetts><Medical><Medical center><Mentors><Microscopy><Mission><Modern Man><Molecular><Molecular Biology><Monkey Pox><Monkeypox><Mutation><NIH><National Institutes of Health><Pattern><Pediatric Hospitals><Ph.D.><PhD><Phenotype><Procedures><Protocol><Protocols documentation><Public Health Schools><RNA Seq><RNA sequencing><RNAseq><Recommendation><Research><Research Design><Research Personnel><Research Specimen><Researchers><SARS corona virus 2><SARS-CO-V2><SARS-COVID-2><SARS-CoV-2><SARS-CoV2><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Sampling><Scanning><Science><Services><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome related corona virus 2><Site><Slide><Software><Sorting><Specimen><Standardization><Structure><Study Type><Targeted Resequencing><Techniques><Technology><Time><Tissue imaging><Tissues><Training><United States National Institutes of Health><Universities><Vaccine Design><Viral><Virus-HIV><Woman><Wuhan coronavirus><biological material><bisulfite sequencing><bisulfite-seq><career><cell culture><cell cultures><cell sorting><chromatin immunoprecipitation-sequencing><co-infection><co-morbid><co-morbidity><coinfection><comorbidity><computational methodology><computational methods><computer based method><computer methods><computing method><conference><convention><coronavirus disease 2019 virus><coronavirus disease-19 virus><data exchange><data integration><data sharing><data transfer><data transmission><develop a vaccine><develop therapy><develop vaccines><development of a vaccine><developmental><epigenetically><flow cytophotometry><genome mutation><genome wide methylation><genomewide methylation><global methylation><hCoV19><image evaluation><image interpretation><imaging><indexing><instrumentation><intervention development><machine based learning><medical college><medical schools><member><mpox><nCoV2><neutralizing antibody><new technology><novel technologies><on-line community><online community><peripheral blood><school of medicine><single cell genomics><study design><summit><super high resolution><superresolution><superresolution microscopy><symposia><symposium><synergism><theories><therapeutic agent development><therapeutic development><therapy development><tool><training opportunity><transcriptome sequencing><transcriptomic sequencing><transcriptomics><treatment development><ultra high resolution><vaccine development><virtual community><virus host interaction>