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Principal Investigator: Mark J Mandel
Organization: UNIVERSITY OF WISCONSIN-MADISON
Fiscal Year: 2024
Award: $56,926
Funding agency: National Institute of General Medical Sciences
PARENT AWARD R35GM148385 PROJECT SUMMARY
The objective of my laboratory is to characterize how molecular communication between bacteria and their
animal hosts leads to specific and reproducible colonization. To accomplish this goal, the laboratory studies the
Vibrio fischeri-squid system, in which the animal’s “light organ” is colonized exclusively by one bacterial
species. This system is advantageous because bacteria colonize through the natural route of infection, all
animals are colonized within three hours of bacterial inoculation into the seawater, the bacteria can be subject
to detailed genetic manipulation, the precise site of infection can be imaged directly in the live animal host, and
chemical analysis of the animal host enables detailed molecular investigations. Focusing on how squid are
reproducibly colonized by the specific symbiont, to the exclusion of the millions of competing bacteria in
seawater, has revealed key roles for bacterial aggregation and biofilm formation in promoting specific host-
microbe interactions. Questions that our group is asking include: (1) How does a symbiont regulate a
beneficial biofilm? Biofilms provide microbes with a protected environment in which they can act collectively
and resist innate immune insults and antimicrobial compounds. V. fischeri elaboration of a symbiotic biofilm is
required for entry into the host, providing an opportunity to study this process in the context of a natural host
colonization model. Our past work identified BinK as a key negative regulator of biofilm formation and the
planktonic-to-biofilm transition in the host. In this study, we examine how BinK interprets signals from the host
and how that information is transmitted to V. fischeri. We examine mechanisms of signal transduction and seek
to identify and characterize a ligand that regulates BinK activity. (2) What novel bacterial factors play critical
functions in colonization processes? We have had success in applying global genetic approaches to
identify bacterial colonization factors in V. fischeri. With a focus on novel and understudied bacterial genes for
which the V. fischeri-squid system has the potential to elucidate protein functions, we identified a protein that
has a substantial impact on biofilm formation and squid colonization. The protein is annotated as a putative
RNA-binding protein, and we will characterize the molecular mechanisms by which this protein acts and
determine how it impacts symbiotic biofilm formation. (3) How do small molecules influence microbiome
specificity and colonization? We have begun to identify compounds that are present in the host and that are
co-regulated with symbiotic behaviors. We will integrate genetic approaches to elucidate signaling pathways in
the context of host colonization. A major strength of the V. fischeri-squid system is the ability to interrogate
bacterial behavior in the intact animal host, and completion of these projects will enable a deeper
understanding of the mechanisms underlying animal colonization by beneficial microbes.
Terms: <Address><Animals><Bacteria><Bacterial Genes><Behavior><Biologic Models><Biological Models><Cell Body><Cell Communication and Signaling><Cell Signaling><Cells><Chemicals><Communication><Environment><Exclusion><Genetic analyses><Goals><Hour><Image><Immune><Immunes><Individual><Infection><Intracellular Communication and Signaling><Investigation><Laboratories><Laboratory Study><Life Style><Lifestyle><Ligands><Light><Microbe><Microbial Biofilms><Model System><Modeling><Molecular><Organ><Photoradiation><Play><Probiotics><Process><Proteins><RNA-Binding Proteins><Reproducibility><Route><Sea Water><Seawater><Signal Pathway><Signal Transduction><Signal Transduction Systems><Signaling><Site><Specificity><Squid><System><Therapeutic Intervention><Transmission><V fischeri><V fischeri-squid system><V. fischeri><V. fischeri-squid system><Vibrio fischeri><Work><anti-microbial><antimicrobial><beneficial flora><beneficial microbes><beneficial microflora><beneficial microorganism><biofilm><biological signal transduction><gene manipulation><genetic analysis><genetic approach><genetic manipulation><genetic strategy><genetically manipulate><genetically perturb><host colonization><host microbe association><host microbe relationship><host-microbe interactions><host-microbial interactions><host-microorganism interactions><imaging><intervention therapy><microbe pathogen><microbial pathogen><microbiome><novel><parent award><parent project><pathogenic microbe><protein function><small molecule><success><symbiont><transmission process><vibrio-squid system>