Document text
Principal Investigator: Jay Kenneth Fisher
Organization: REDBUD LABS, INC.
Fiscal Year: 2020
Award: $299,268
Funding agency: National Institute of Allergy and Infectious Diseases
ABSTRACT
We propose to develop a point-of-need system for differential molecular identification of filoviruses from
syndromically similar infections. Our goal is to enable rapid, sensitive, and specific identification of quarantinable
infections to reduce nosocomial risk and improve outbreak response. The platform will simultaneously test 12
genomic markers at the genus and strain level using a modular microfluidic design that allows for rapid panel
update and expansion. In Phase I we will develop an assay for pan-filovirus (pFi), followed by expansion of the
panel and system integration in Phase II. The expanded panel will add pan-flavivirus (pFa) and pan-Plasmodium
(pPa) detection as well as strain-specific targets for Ebola, Marburg, Dengue, Yellow Fever, and Malaria. Strain
specific tests will enable better patient triage/treatment during an outbreak and improve disease surveillance in
non-outbreak settings.
The panel will require a 50 μL sample of whole blood and will achieve highly specific detection in < 30 minutes.
To achieve this goal, we will combine Redbud Labs’ expertise in microfluidics and systems with the diagnostic
development expertise of the Diagnostics Program at PATH (Seattle, WA), and the VHF testing/processing
capabilities of the Connor Lab at Boston University (part of the BSL-4 facilities at NEIDL).
Terms: <Africa><Animal Model><Animal Models and Related Studies><Assay><Automation><BSL-4 facility><BSL4 facility><Bioassay><Biologic Assays><Biological Assay><Blood><Blood Reticuloendothelial System><Blood Sample><Blood specimen><Boston><Breakbone Fever Virus><Buffers><Chimp><Chimpanzee><Clinical><Communicable Diseases><Confusion><Confusional State><Consensus Sequence><Cytolysis><Dangerousness><Decision Making><Dengue><Dengue Fever><Dengue Virus><Dengue disease><Dengue fever virus><Deposit><Deposition><Detection><Detergents><Development><Devices><Diagnosis><Diagnostic><Differential Diagnosis><Disease><Disease Outbreaks><Disease Surveillance><Disorder><EBOV><Ebola><Ebola virus><Epidemiologic Monitoring><Epidemiologic surveillance><Epidemiological Monitoring><Epidemiological surveillance><Epidemiology Surveillance><Failure><Family><Filoviridae><Filoviridae Infections><Filovirus><Flavivirus><Frankfurt-Marburg Syndrome Virus><Freeze Drying><Freeze Dryings><Goals><Group B Arbovirus><Health Resources><Health system><Hospital Infections><Hospital acquired infection><Infection><Infectious Disease Pathway><Infectious Diseases><Infectious Disorder><Infrastructure><Laboratories><Lyophilization><Lysis><Malaria><Marburg><Marburg virus><Measures><Mental Confusion><Methods><Microfluidics><Molecular><Non-Polyadenylated RNA><Nosocomial Infections><Nucleic Acids><Outbreaks><P falciparum><P. falciparum><P.falciparum><Paludism><Pan Genus><Pan Species><Parasites><Patient Triage><Patients><Performance><Phase><Plasmodium><Plasmodium Infections><Plasmodium falciparum><Public Health><Quarantine><RNA><RNA Gene Products><Reagent><Research><Response Elements><Ribonucleic Acid><Risk><Running><Sampling><Sensitivity and Specificity><Specificity><Spottings><Symptoms><Syndrome><System><Systems Integration><Test Result><Testing><Time><Triage><Universities><Update><Validation><Viral Hemorrhagic Fevers><Virus><Whole Blood><Yellow Fever><Yellow fever virus><assay development><base><biosafety level 4 facility><breakbone fever><care providers><cost><cross reactivity><design><designing><developmental><diagnostic assay><ebolavirus><falls><filovirus infections><genomic biomarker><genomic marker><hemorrhagic fever><high risk><improved><infection risk><innovate><innovation><innovative><institutional infection><isolation/quarantine><microfluidic technology><model of animal><model organism><molecular diagnostics><mortality><patient population><pre-clinical><preclinical><primary care provider><programs><response><success><tissue culture><viron><µfluidic><µfluidic technology>