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Principal Investigator: Barbara Lipska
Organization: NATIONAL INSTITUTE OF MENTAL HEALTH
Fiscal Year: 2021
Award: $3,811,597
Funding agency: National Institute of Mental Health
Currently, the Human Brain Collection Core (HBCC) has 1238 brains available for distribution, with the following diagnoses (numbers of subjects listed in parentheses): attention deficit disorder (21); anxiety disorders (25); autism spectrum disorders (3); Bipolar disorder (156), Non-psychiatric controls (325), severe psychotic disorders (including schizophrenia and schizoaffective disorder: 188), substance use disorders (109), Major depressive disorder (270), post-traumatic stress disorder (13), obsessive compulsive disorder (12), Other psychiatric diagnoses (49), Undetermined (21). In 42 cases, the diagnosis is still pending. Three cases with neurological disorders will be transferred to other banks.
From Oct 1, 2020, until August 5, 2021 we collected 40 brains through Northern and Central Virginias Medical Examiners Offices.
Other resources include:
-cDNA libraries constructed from dorsolateral prefrontal cortex (DLPFC), hippocampus, anterior cingulate cortex (ACC) and subgenual anterior cingulate cortex (sgACC), and dura from hundreds of subjects with psychiatric disorders and controls
-microarray expression and genotyping data (publicly available at the NIMH data archive (NDA: https://nda.nih.gov/edit_collection.html?id=3151) from DLPFC, hippocampus and dura
-frozen sections (14 um thick) mounted on slides (DLPFC from 32 patients with schizophrenia and 63 controls),
-formalin-fixed coronal slices (approximately 15 mm thick) of a single hemisphere from 15 controls, 10 patients with schizophrenia, 5 with major depression, 4 with bipolar disorder.
-Fibroblasts derived from postmortem dura: 450
-Whole genome sequencing, RNA sequencing, and Chip-sequencing (acetylation and methylation marks) data for DLPFC of 400 subjects (publicly available at NDA: https://nda.nih.gov/edit_collection.html?id=3151), on the CommonMind Consortium portal and on the PsychEncode resource page: http://resource.psychencode.org/)
-RNA sequencing data from 200 individuals from the subgenual ACC, 219 from the Superior Temporal Gyrus and 311 from the dorsal anterior cingulate are also available on NDA (https://nda.nih.gov/edit_collection.html?id=3151).
We share information with Neurobiobank (NBB). Our inventory of cases can be searched through the Neurobiobank website (https://neurobiobank.nih.gov/). We obtain detailed quantitative electronic toxicological data on hundreds of our specimens and share them with NBB. We use international classification of diseases (ICD10) codes for diagnostic definitions.
In the last year, we have responded to 47 requests for tissue or data, including 18 from NIH, 8 letters of support for grant applications, and 21 requests from other institutions. We have distributed 2533 tissue samples this year, including 829 to NIH and 1704 to other institutions.
We now characterize COVID-19 status in all cases received by a naso-pharingeal swab of the cadaver and serological testing. We have identified three cases positive for Sars-CoV2 with recent infection and four more with evidence of past infection. All were asymptomatic or pauci-symptomatic at the time of their death.
Some of the tissue distribution requests that are still in progress or have been initiated in the last year are:
1. Single-nucleus differential gene expression in schizophrenia
2. Whole genome sequencing with Illumina technology of 809 HBCC cases
3. snRNA-seq and bulk RNA-seq profiling for carriers of high-risk structural and copy-number variants
4. Optimization of dissection of the mediodorsal thalamus, for genomics and histological characterization
5. Sex related differences in cell composition of the human cortex
6. Investigation of cerebrovascular micro-damage in major depressive disorder
7. Dynorphin/kappa-opioid receptor regulation of prefrontal cortical circuitry
8. RNA-seq and methylomics in Attention Deficit Disorder
9. Mapping gene expression in PD-relevant human brain regions
10. Mapping gene expression and cell populations during healthy aging
11. Neuropathology of COVID
12. Genetic characterization of neurodegenerative dementias
13. Long-read DNA sequencing of human brain tissue
14. Gene expression and protein analysis from spinal bulbar and muscular atrophy patients and controls
15. Post-Mortem Brain Anatomy of Chronic Pain and Fatiguing Disorders
16. Astrovirus encephalitis in immunocompromised humans
17. 3D genomic characterization of autism and schizophrenia
18. Integrated, cell type specific functional genomics analyses of regulatory sequence elements in neuropsychiatric brain tissues
19. Fine-Mapping Genome-Wide Associated Loci using Multi-omics Data to Identify Mechanisms Affecting Serious Mental Illness
20. RNA sequencing of single nuclei from postnatal and adult human neural progenitors
21. Molecular and cellular profiling of spoken-language brain circuits in humans
22. Maturation of psychiatric circuits
23. Identifying the differences in single-cell transcriptomes between schizophrenia and bipolar disorder in the anterior cingulate cortex
24. Mapping the role of long noncoding RNAs in gene regulatory networks in schizophrenia
25. Creating a resource of genetic variables for postmortem brains in the Human Brain Collection Core (HBCC)
26. Human Brain Plasticity Across the Life Span
27. Utilizing Single-Nucleus Sequencing to Understand APOE Biology in the Brain
28. Profiling cell-type transcriptional changes induced by chronic cannabis exposure
29. Characterization of postmortem brain and blood inflammatory transcriptomic overlaps in mood disorder
30. Unraveling the molecular mechanisms of myelination deficits in Schizophrenia
31. Diagnostic Use of Exosomal mRNA
32. Investigating Schizophrenia Brain Subtypes using PET Sphingosine-1-Phosphate Receptor-1 (S1PR1) Imaging
33. Association of SARS-CoV-2 infection and Alzheimer's-like neuropathology
Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><21+ years old><3-D><3-Dimensional><3D><AD dementia><Acetylation><Adult><Adult Human><Affect><Affective Disorders><Alzheimer><Alzheimer Type Dementia><Alzheimer disease><Alzheimer sclerosis><Alzheimer syndrome><Alzheimer's><Alzheimer's Disease><Alzheimer's disease dementia><Alzheimers Dementia><Alzheimers disease><Ammon Horn><Anatomic><Anatomic Sites><Anatomic structures><Anatomical Sciences><Anatomy><Anterior><Anxiety Disorders><Applications Grants><Astrovirus><Attention Deficit Disorder><Audiotape><Authorization><Authorization documentation><Autism><Autistic Disorder><Autopsy><Biology><Bipolar Affective Psychosis><Bipolar Disorder><Blood><Blood Reticuloendothelial System><Blood Sample><Blood specimen><Body Tissues><Brain><Brain Inflammation><Brain Nervous System><Brain region><COVID><COVID-19><COVID-19 infection><COVID-19 virus><COVID19><COVID19 infection><COVID19 virus><CV-19><CV19><Cadaver><Cannabis><Cell Body><Cell Nucleus><Cells><Cessation of life><ChIP Sequencing><ChIP-seq><Chromosome Mapping><Chronic><Clinical><CoV disease><CoV-2><CoV2><Code><Coding System><Collection><Communities><Consent><Copy Number Polymorphism><Cornu Ammonis><D.C. Washington><DC Washington><DNA><Data><Data Bases><Databases><Death><Deoxyribonucleic Acid><Development><Diagnosis><Diagnostic><Differences between sexes><Differential Gene Expression><Differs between sexes><Disease><Disorder><Dissection><District of Columbia><Dorsal><Drug or chemical Tissue Distribution><Dura><Dura Mater><Dynorphins><Early Infantile Autism><Edg Receptors><Elements><Encephalitis><Encephalon><Ensure><Equipment and supply inventories><Evaluation><Family><Fatigue><Fibroblasts><Formalin><Freeze Sectioning><Frozen Sections><Functional RNA><Gene Expression><Gene Localization><Gene Mapping><Gene Mapping Genetics><Gene Proteins><Gene Transcription><Genetic><Genetic Transcription><Genetic study><Genome Mappings><Genomics><Genotype><Goals><Grant Proposals><Hair><Hippocampus><Hippocampus (Brain)><Histologic><Histologically><History><Human><Human Resources><Image><Immunocompromised><Immunocompromised Host><Immunocompromised Patient><Immunosuppressed Host><Individual><Infantile Autism><Infection><Inflammatory><Institution><International Classification of Diseases><International Statistical Classification of Diseases and Related Health Problems><Interview><Intramural Program><Intramural Research Program><Inventory><Investigation><Investigators><Kanner's Syndrome><Label><Lack of Energy><Language><Length of Life><Letters><Linkage Mapping><Longevity><Low Molecular Weight Nuclear RNA><Major Depressive Disorder><Major Mental Illness><Manic-Depressive Psychosis><Manpower><Medical><Medical Examiners><Medical Records><Mental Depression><Mental Health><Mental Hygiene><Mental disorders><Mental health disorders><Messenger RNA><Methylation><Mission><Modern Man><Molecular><Molecular Genetics><Mood Disorders><Multiomic Data><NIH><NIMH><National Institute of Mental Health><National Institutes of Health><Nervous System Diseases><Neural Stem Cell><Neurologic Disorders><Neurological Disorders><Non-Coding><Non-Coding RNA><Non-Polyadenylated RNA><Non-translated RNA><Noncoding RNA><Nontranslated RNA><Nucleus><Obsessive-Compulsive Disorder><Obsessive-Compulsive Neurosis><PET><PET Scan><PET imaging><PETSCAN><PETT><PTSD><Patients><Permission><Population><Positron Emission Tomography Medical Imaging><Positron Emission Tomography Scan><Positron-Emission Tomography><Post-Traumatic Neuroses><Post-Traumatic Stress Disorders><Posttraumatic Neuroses><Posttraumatic Stress Disorders><Prefrontal Cortex><Preventative strategy><Prevention strategy><Preventive strategy><Primary Senile Degenerative Dementia><Protein Analysis><Protein Gene Products><Psychiatric Diagnosis><Psychiatric Disease><Psychiatric Disorder><Psychological Health><Psychotic Disorders><RNA><RNA Expression><RNA Gene Products><RNA Seq><RNA sequencing><RNAseq><Rad.-PET><Recording of previous events><Regulation><Regulator Genes><Reporting><Research><Research Personnel><Research Resources><Research Specimen><Research Support><Researchers><Resources><Ribonucleic Acid><Role><S1P Receptor><SARS corona virus 2><SARS-CoV-2><SARS-CoV-2 infection><SARS-CoV2><SARS-CoV2 infection><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Sampling><Schizoaffective Disorders><Schizophrenia><Schizophrenic Disorders><Serology test><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome coronavirus 2 infection><Severe acute respiratory syndrome related corona virus 2><Sex Differences><Sexual differences><Slice><Slide><Small Molecular Weight RNA><Small Nuclear RNA><Specimen><Sphingosine-1-Phosphate Receptor><Structure><Substance Use Disorder><Substance abuse problem><Suicide><Superior temporal gyrus><Swab><Synapses><Synaptic><Technology><Thalamic structure><Thalamus><Thick><Thickness><Time><Tissue Distribution><Tissue Sample><Tissue-Specific Differential Gene Expression><Tissue-Specific Gene Expression><Tissues><Total Human and Non-Human Gene Mapping><Toxicology><Transcription><Transcriptional Regulatory Elements><United States National Institutes of Health><Untranslated RNA><Virginia><Wuhan coronavirus><abuse of substances><adulthood><autism spectrum disorder><autistic spectrum disorder><bipolar affective disorder><bipolar disease><bipolar illness><brain behavior><brain dysfunction><brain tissue><cDNA Library><cell type><cerebral vascular><cerebro-vascular><cerebrovascular><chromatin immunoprecipitation-sequencing><chronic mental illness><chronic pain><cingulate cortex><clinical depression><copy number variant><copy number 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span><lifespan><mRNA><major depression><major depression disorder><manic depressive disorder><manic depressive illness><mental illness><methylomics><multiple omic data><myelination><nCoV2><necropsy><nerve stem cell><nervous system disorder><neural precursor><neural precursor cell><neural progenitor><neural progenitor cells><neurodegenerative dementia><neurological disease><neuron progenitors><neuronal progenitor><neuronal progenitor cells><neuronal stem cells><neuropathology><neuroprogenitor><neuropsychiatric><neuropsychiatry><new drug treatments><new drugs><new therapeutics><new therapy><next generation therapeutics><noncoding><novel drug treatments><novel drugs><novel therapeutics><novel therapy><persistent mental illness><personnel><positron emission tomographic (PET) imaging><positron emission tomographic imaging><positron emitting tomography><post-natal human><post-trauma stress disorder><postmortem><postnatal human><posttrauma stress disorder><primary degenerative dementia><psychiatric illness><psychological disorder><psychotic illness><regulatory gene><sample collection><schizophrenic><senile dementia of the Alzheimer type><serious mental disorder><serious mental illness><serology assay><severe mental disorder><severe mental illness><sex-dependent differences><sex-related differences><sex-specific differences><snRNA><social role><specimen collection><spinal and bulbar muscular atrophy><substance abuse><suicidality><synapse><thalamic><three dimensional><trans acting element><transcriptome><transcriptome sequencing><transcriptomics><traumatic neurosis><uRNA><web site><website><whole genome><κ opiate><κ opioid><κ opioid receptors><κ-OR><κOR>