Document text
Principal Investigator: Dirk P Dittmer
Organization: UNIV OF NORTH CAROLINA CHAPEL HILL
Fiscal Year: 2024
Award: $233,250
Funding agency: National Institute of Dental and Craniofacial Research
ABSTRACT
Clinical virology is predicated on the workflow of analyzing small sample volumes (to minimize patient discomfort,
storage, and fit legacy infrastructure) for a single analyte, i.e., virus, with utmost precision. The human virome
project (HVP) does not share these restrictions. The HVP priority tissues of urine, stool, saliva, and blood can
be acquired from healthy people in a 50 – 500 ml sample size. For instance, the Red Cross makes normal
plasma available in 500 ml bags, and NGS allows for the characterization of all human viruses in a single
reaction. This proposal responds to RFA-RM-23-018 with the development of two novel tools: AIM 1, a virus
purification pipeline that utilizes larger volumes to achieve < 1 genome copy/ ml sensitivity; AIM 2, a single virion
detection pipeline to provide an accurate denominator of how many intact virions a given NGS sequence set is
representing. We hypothesize that Viral Direct Counts (VDC) akin to bacterial direct counts will become the
essential parameter for integrating the studies in the HVP.
Terms: <2019-nCoV S protein><2019-nCoV spike glycoprotein><2019-nCoV spike protein><Address><Advanced Instrumentation><Antigens><Assay><Aujeszky's Disease Virus><Aujeszkys Disease Virus><Back><Bio-Informatics><Bioassay><Bioinformatics><Biological Assay><Biomass><Blood><Blood Plasma><Blood Reticuloendothelial System><Blossoms><Body Tissues><CLIA accredited><CLIA approved><CLIA certified><CLIA compliant><CLIA licensed><COVID-19 S protein><COVID-19 spike><COVID-19 spike glycoprotein><COVID-19 spike protein><Clinic Visits><Clinical Microbiology><Clinical Virology><Collaborations><Communities><Computer Instrumentation><Computers and Advanced Instrumentation><Cryo-electron Microscopy><Cryoelectron Microscopy><DNA><Dedications><Deoxyribonucleic Acid><Detection><Development><Diagnostic><Dorsum><Electron Cryomicroscopy><Elements><Ensure><Environment><Face><Feces><Flowers><Fluorescence><Funding><Genome><Genomics><Goals><Head><Health><Herpesviridae><Herpesvirus 1 (alpha), Suid><Herpesvirus Suis><Herpesviruses><Heterogeneity><Hospitals><Human><Image><Individual><Infrastructure><Laboratories><Manufacturer><Measures><Methods><Modern Man><Mouthwash><NGS Method><NGS system><Nucleic Acids><Outcome><Participant><Patients><Persons><Plant Blooms><Plasma><Plasma Serum><Proteins><Pseudorabies virus><Reaction><Red Cross><Reference Standards><Reproducibility><Research><Research Specimen><Reticuloendothelial System, Serum, Plasma><Running><SARS-CoV-2 S><SARS-CoV-2 S protein><SARS-CoV-2 spike><SARS-CoV-2 spike glycoprotein><SARS-CoV-2 spike protein><Saliva><Sample Size><Sampling><Severe acute respiratory syndrome coronavirus 2 S protein><Severe acute respiratory syndrome coronavirus 2 spike glycoprotein><Severe acute respiratory syndrome coronavirus 2 spike protein><Specimen><Standardization><Suid Herpesvirus 1><Swine Herpesvirus 1><System><Time><Tissues><Transmission Electron Microscopy><Ultracentrifugation><Urine><Viral><Viral Burden><Viral Load><Viral Load result><Virion><Virus><Virus Particle><Visualization><clinical diagnostics><comparative><coronavirus disease 2019 S protein><coronavirus disease 2019 spike glycoprotein><coronavirus disease 2019 spike protein><cryo-EM><cryoEM><cryogenic electron microscopy><design><designing><detection method><detection procedure><detection technique><developmental><digital><entire genome><experience><faces><facial><full genome><genome sequencing><herpes virus><human tissue><human virome><imaging><immunogen><innovate><innovation><innovative><mad itch virus><medical college><medical diagnostic><medical schools><molecular biomarker><molecular marker><nano particle><nano-sized particle><nanoparticle><nanosized particle><next gen sequencing><next generation sequencing><nextgen sequencing><novel><particle><programs><school of medicine><spike proteins on SARS-CoV-2><statistics><stool><success><superresolution microscopy><timeline><tool><tool development><viral genomics><viral microbiome><virome><virus culture><virus genomics><volunteer><whole genome>