Coronavirus Challenge Core

NIH Pandemic-Era Grants

Pandemic Era Grants

2022

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Principal Investigator: Matthew Bryan Frieman
Organization: WASHINGTON UNIVERSITY
Fiscal Year: 2022
Award: $1,441,507
Funding agency: National Institute of Allergy and Infectious Diseases

Project Summary – Animal Challenge Core B
The Animal Challenge Core B will develop and provide key virus stocks for four different human
and animal coronaviruses (CoVs) that have pandemic potential. These viruses belong to the
subgenera of Sarbecovirus and Merbecovirus and will be used by the Core for challenge studies
in mice and Syrian hamsters to evaluate cross-protective efficacy of vaccines against diverse
betacoronaviruses. The virus stocks, generated by Core B, also will be used in virus neutralization
assays prior to challenge or after immunogenicity testing of vaccines in mice and hamsters. The
specific functions of Core B will include: (a) generating validated stocks of four different
betacoronaviruses including SARS-CoV-1, SARS-CoV-2, SHC-014 and pangolin/MP789/2019;
(b) developing rodent models for bat coronaviruses; (c) Contributing to and performing pivotal
immunogenicity studies in mice and hamsters to quantify the B and T cell response to vaccination
with different vaccine candidates generated in Projects 1-3; (d) Performing virus challenge
studies with human and bat CoV in mice and hamsters immunized with candidate vaccines that
induce broadly reactive B- and T-cell responses to diverse betacoronaviruses. The results from
Core B will be used by the data management team in Core A to identify correlates of immune
cross-protection against betacoronaviruses of pandemic concern. These functons will enable the
PPG group to determine whether a candidate vaccine induces broadly protective immunity
against human and animal CoV. This Core B, led by Drs. Frieman and Boon, will meet the needs
of the PPG by acquiring and generating challenge viruses, developing animal models and
performing immunogenicity and vaccine challenges studies in established rodent models of
betacoronaviruses. These functions provide key infrastructure support for all research projects of
the PPG.

Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><ACE2><Ad vector><Adenoviral Vector><Adenovirus Vector><Animal Model><Animal Models and Related Studies><Animals><Antibodies><Antibody Response><Antigenic Determinants><Antisera><Assay><Attenuated Live Virus Vaccine><B blood cells><B cell><B cells><B-Cells><B-Lymphocytes><B-cell><Bats><Binding><Binding Determinants><Bioassay><Biologic Assays><Biological Assay><Blood Serum><Body Weight decreased><COVID-19 virus><COVID19 virus><Cell Body><Cell Line><CellLine><Cells><Chimp><Chimpanzee><Chiroptera><CoV-2><CoV2><Coronaviridae><Coronavirus><Cricetinae><Development><Dose><ELISA><Engineering><Ensure><Enzyme-Linked Immunosorbent Assay><Epitopes><Evaluation><Flow Cytofluorometries><Flow Cytofluorometry><Flow Cytometry><Flow Microfluorimetry><Flow Microfluorometry><Goals><Golden Hamsters><Golden Syrian Hamsters><HCoV><Hamsters><Hamsters Mammals><Human><Immune><Immune Sera><Immunes><Immunity><Immunize><Immunochemical Immunologic><Immunologic><Immunological><Immunologically><Immunologics><Individual><Inflammation><Infrastructure><Laboratories><MERS corona virus><MERS coronavirus><MERS virus><MERS-CoV><Merbecovirus><Mesocricetus auratus><Methods><Mice><Mice Mammals><Middle East Respiratory Syndrome Corona Virus><Middle East Respiratory Syndrome Coronavirus><Middle East Respiratory Syndrome Virus><Middle East Respiratory Syndrome-CoV><Middle Eastern Respiratory Syndrome Corona virus><Middle Eastern Respiratory Syndrome Coronavirus><Middle Eastern Respiratory Syndrome Virus><Middle Eastern Respiratory Syndrome-CoV><Modern Man><Molecular Interaction><Murine><Mus><P01 Mechanism><P01 Program><PBMC><Pan Genus><Pan Species><Pathologic><Pathology><Peripheral Blood Mononuclear Cell><Program Project Grant><Program Research Project Grants><Proteins><Receptor Protein><Research><Research Program Projects><Rodent Model><SARS Virus><SARS corona virus><SARS corona virus 2><SARS coronavirus><SARS-Associated Coronavirus><SARS-CO-V2><SARS-COVID-2><SARS-CoV><SARS-CoV-1><SARS-CoV-2><SARS-CoV2><SARS-Related Coronavirus><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Sarbecovirus><Serum><Severe Acute Respiratory Coronavirus><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome Virus><Severe Acute Respiratory Syndrome corona virus><Severe Acute Respiratory Syndrome coronavirus><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome related corona virus 2><Spleen><Spleen Reticuloendothelial System><Strains Cell Lines><Surface><Syrian Hamsters><T cell response><T-Cells><T-Lymphocyte><Testing><Transgenic Mice><Transgenic Organisms><VSV><Vaccinated><Vaccination><Vaccine Antigen><Vaccines><Vesicular Stomatitis Virus><Vesicular stomatitis Indiana virus><Viral><Viral load measurement><Virus><Weight Loss><Weight Reduction><Wuhan coronavirus><adeno vector><adenovector><angiotensin converting enzyme 2><angiotensin converting enzyme II><animal coronavirus><base><beta CoV><beta coronavirus><betaCoV><betacoronavirus><body weight loss><corona virus><coronavirus disease 2019 virus><coronavirus disease-19 virus><cross immunity><cross protection><cross reactivity><cultured cell line><data management><design><designing><developmental><enzyme linked immunoassay><evaluate vaccines><experiment><experimental research><experimental study><flow cytophotometry><hCoV19><human CoV><human corona virus><human coronavirus><immune serum><immunogenicity><model of animal><model organism><nCoV2><pan CoV vaccine><pan coronavirus vaccine><panCoV vaccine><pancoronavirus vaccine><pandemic><pandemic disease><pre-clinical><preclinical><protective efficacy><receptor><receptor binding><receptor bound><severe acute respiratory syndrome-CoV><thymus derived lymphocyte><transgenic><universal CoV vaccine><universal coronavirus vaccine><vaccine candidate><vaccine efficacy><vaccine evaluation><vaccine screening><vaccine testing><virus load><wt-loss><β CoV><β coronavirus><βCoV>