Document text
Principal Investigator: Suzie H. Pun
Organization: UNIVERSITY OF WASHINGTON
Fiscal Year: 2022
Award: $455,938
Funding agency: National Institute on Alcohol Abuse and Alcoholism
PROJECT SUMMARY
The SARS-CoV-2 coronavirus, the cause of the COVID-19 global pandemic, is efficiently spread
and has reached over 27 million confirmed cases as of September 8, 2020. There is therefore an
urgent need for new technologies that can provide early detection of virus, reducing the
transmission and infection rate. The goal of this proposal is to develop an integrated biosensor-
touchscreen that sensitivity reports surface contact with SARS-CoV-2. In our preliminary work,
we have identified several aptamers that bind specifically to the envelope-anchored trimeric spike
(S) protein of SARS-CoV-2, but not of SARS-CoV or MERS. In comparison to antibodies,
aptamers are synthetic molecules that more thermally stable and lower cost while providing
similar specificity and affinity of target binding. In this application, we propose to integrate
aptamer-based biosensing of SARS-CoV-2 into a touchscreen device. Our main objectives are to
1) engineer conformation switching aptamers for electrochemical sensing of SARS-CoV-2
binding, 2) develop nanogap capacitive sensors as a uniquely complementary approach to
capacitive touchscreen technology and 3) build and test an integrated biosensor and touchscreen
array that can detect SARS-CoV-2 from patient samples. Successful completion of these aims
will result in a novel automatic sensing platform for SARS-CoV-2. This technology could transform
personal device touchscreens as well as to multi-user touchscreen devices in hospitals, airports,
libraries, restaurants, for early detection, curbing transmission rates from secondary exposure.
Importantly, the developed technology could be adapted for other electronic sensing platforms,
and easily applied for future pathogen detection.
Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><2019-nCoV S protein><2019-nCoV spike glycoprotein><2019-nCoV spike protein><Affinity><Air><Antibodies><Binding><Binding Proteins><Biosensing Technics><Biosensing Techniques><Biosensor><COVID crisis><COVID epidemic><COVID infected patient><COVID pandemic><COVID patient><COVID positive patient><COVID-19><COVID-19 S protein><COVID-19 crisis><COVID-19 detection><COVID-19 epidemic><COVID-19 global health crisis><COVID-19 global pandemic><COVID-19 health crisis><COVID-19 infected patient><COVID-19 pandemic><COVID-19 patient><COVID-19 positive patient><COVID-19 public health crisis><COVID-19 spike glycoprotein><COVID-19 spike protein><COVID-19 transmission><COVID-19 virus><COVID-19 virus transmission><COVID19><COVID19 detection><COVID19 S protein><COVID19 crisis><COVID19 epidemic><COVID19 global health crisis><COVID19 global pandemic><COVID19 health crisis><COVID19 pandemic><COVID19 patient><COVID19 positive patient><COVID19 public health crisis><COVID19 spike glycoprotein><COVID19 spike protein><COVID19 virus><CV-19><CV19><Car Phone><Cell Communication and Signaling><Cell Phone><Cell Signaling><Cellular Phone><Cellular Telephone><Cessation of life><Chemistry><Clinical><CoV-2><CoV2><Communicable Disease Contact Tracing><Contact Tracing><Coronaviridae><Coronavirus><Death><Detection><Development><Devices><Disease Outbreaks><Early Diagnosis><Electric Capacitance><Electrical Capacitance><Electrodes><Electronics><Engineering><Evaluation><Exhibits><Face><Frequencies><Future><Glass><Goals><Hospitals><Immobilization><Individual><Infection><Infectious Disease Contact Tracing><Intracellular Communication and Signaling><Label><Libraries><Ligand Binding Protein><Ligand Binding Protein Gene><MERS><MERS coronavirus disease><MERS-CoV disease><Measures><Middle East Respiratory Syndrome><Middle East Respiratory Syndrome CoV disease><Middle East Respiratory Syndrome coronavirus disease><Middle Eastern Respiratory Syndrome><Middle Eastern Respiratory Syndrome CoV disease><Middle Eastern Respiratory Syndrome coronavirus disease><Mobile Phones><Molecular Configuration><Molecular Conformation><Molecular Interaction><Molecular Stereochemistry><Natural regeneration><Outbreaks><Oxidation-Reduction><Pathogen detection><Pattern><Performance><Preparation><Process><Protein Binding><Redox><Regeneration><Reporting><Restaurants><SARS Virus><SARS corona virus><SARS corona virus 2><SARS coronavirus><SARS-Associated Coronavirus><SARS-CO-V2><SARS-COVID-2><SARS-CoV><SARS-CoV-1><SARS-CoV-2><SARS-CoV-2 S protein><SARS-CoV-2 detection><SARS-CoV-2 epidemic><SARS-CoV-2 global health crisis><SARS-CoV-2 global pandemic><SARS-CoV-2 infected patient><SARS-CoV-2 pandemic><SARS-CoV-2 patient><SARS-CoV-2 positive patient><SARS-CoV-2 spike glycoprotein><SARS-CoV-2 spike protein><SARS-CoV-2 transmission><SARS-CoV2><SARS-CoV2 S protein><SARS-CoV2 epidemic><SARS-CoV2 pandemic><SARS-CoV2 spike glycoprotein><SARS-CoV2 spike protein><SARS-Related Coronavirus><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-coronavirus-2 epidemic><SARS-coronavirus-2 pandemic><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Sampling><Sensitivity and Specificity><Severe Acute Respiratory Coronavirus><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome CoV 2 epidemic><Severe Acute Respiratory Syndrome CoV 2 pandemic><Severe Acute Respiratory Syndrome Virus><Severe Acute Respiratory Syndrome corona virus><Severe Acute Respiratory Syndrome coronavirus><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome coronavirus 2 S protein><Severe acute respiratory syndrome coronavirus 2 epidemic><Severe acute respiratory syndrome coronavirus 2 pandemic><Severe acute respiratory syndrome coronavirus 2 spike glycoprotein><Severe acute respiratory syndrome coronavirus 2 spike protein><Severe acute respiratory syndrome related corona virus 2><Signal Transduction><Signal Transduction Systems><Signaling><Specificity><Surface><System><Technology><Testing><Time><Touch><Touch sensation><Transmission><Vaccines><Validation><Virion><Virus Particle><Work><Wuhan coronavirus><aptamer><base><biological sensor><biological signal transduction><biosensing><bound protein><capacitance><conformation><conformational state><corona virus><corona virus disease 2019><corona virus disease 2019 epidemic><corona virus disease 2019 pandemic><coronavirus disease 2019><coronavirus disease 2019 S protein><coronavirus disease 2019 crisis><coronavirus disease 2019 detection><coronavirus disease 2019 epidemic><coronavirus disease 2019 global health crisis><coronavirus disease 2019 global pandemic><coronavirus disease 2019 health crisis><coronavirus disease 2019 infected patient><coronavirus disease 2019 pandemic><coronavirus disease 2019 patient><coronavirus disease 2019 positive patient><coronavirus disease 2019 public health crisis><coronavirus disease 2019 spike glycoprotein><coronavirus disease 2019 spike protein><coronavirus disease 2019 transmission><coronavirus disease 2019 virus><coronavirus disease 2019 virus transmission><coronavirus disease crisis><coronavirus disease epidemic><coronavirus disease infected patient><coronavirus disease pandemic><coronavirus disease patient><coronavirus disease positive patient><coronavirus disease-19><coronavirus disease-19 global pandemic><coronavirus disease-19 pandemic><coronavirus disease-19 patient><coronavirus disease-19 virus><coronavirus infectious disease-19><coronavirus patient><cost><density><design><designing><detect COVID-19><detect COVID19><detect SARS-CoV-2><detect severe acute respiratory syndrome coronavirus 2><developmental><early detection><electronic device><faces><facial><hCoV19><iPhone><infection rate><instrumentation><nCoV2><nano fabricate><nano fabrication><nanofabricate><nanofabrication><new technology><novel><novel technologies><orthopedic freezing><oxidation reduction reaction><patient infected with COVID><patient infected with COVID-19><patient infected with SARS-CoV-2><patient infected with coronavirus disease><patient infected with coronavirus disease 2019><patient infected with severe acute respiratory syndrome coronavirus 2><patient with COVID><patient with COVID-19><patient with COVID19><patient with SARS-CoV-2><patient with coronavirus disease><patient with coronavirus disease 2019><patient with severe acute respiratory distress syndrome coronavirus 2><prevent><preventing><rate of infection><regenerate><secondary infection><sensor><severe acute respiratory syndrome coronavirus 2 detection><severe acute respiratory syndrome coronavirus 2 global health crisis><severe acute respiratory syndrome coronavirus 2 global pandemic><severe acute respiratory syndrome coronavirus 2 infected patient><severe acute respiratory syndrome coronavirus 2 patient><severe acute respiratory syndrome coronavirus 2 positive patient><severe acute respiratory syndrome coronavirus 2 transmission><severe acute respiratory syndrome-CoV><smart phone><smartphone><tactile sensation><touch panel><touch screen><touch screen panel><touchscreen><touchscreen panel><transmission process><transmitted COVID-19><transmitted SARS-CoV-2><transmitted coronavirus disease 2019><transmitted severe acute respiratory syndrome coronavirus 2><viral detection><viral transmission><virus detection><virus transmission>