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Principal Investigator: Yan Li
Organization: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
Fiscal Year: 2024
Award: $1,011,974
Funding agency: National Institute of Neurological Disorders and Stroke
PCF is equipped with a Thermo Fisher Lumos mass spectrometer with an nanoflow Ultimate 3000 HPLC, a Thermo Fisher Ascend mass spectrometer with a Vanquish NEO HPLC, and an Agilent 6545XT Advance Bio LC/Q-TOF System.
PCF seeks to provide high quality proteomics services on intact Protein molecular weight measurement; protein identification; protein quantification using label-free or labeled approaches; identification of PTMs including phosphorylation, acetylation, ubiquitination, glutamination and disulfide linkage characterization. PCF also develop methods for unique projects.
PCF staff also train users on instrument operation, educate users on mass spectrometry-based techniques, stay informed on new techniques and devices, and make them available to PCF users when possible.
From 08/2023 to 08/2024, PCF supported more than 30 investigators within and outside NINDS, worked on more than 50 projects, co-authored in 7 papers listed below.
1. Reversing anxiety by targeting a stress-responsive signaling pathway.
Pandey S, Han W, Li J, Shepard R, Wu K, Castellano D, Tian Q, Dong L, Li Y, Lu W.Proc Natl Acad Sci U S A. 2024 Jul 30;121(31):e2400078121. doi: 10.1073/pnas.2400078121. Epub 2024 Jul 26.
2. TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression.
Garcia-Montojo M, Fathi S, Rastegar C, Simula ER, Doucet-O'Hare T, Cheng YHH, Abrams RPM, Pasternack N, Malik N, Bachani M, Disanza B, Maric D, Lee MH, Wang H, Santamaria U, Li W, Sampson K, Lorenzo JR, Sanchez IE, Mezghrani A, Li Y, Sechi LA, Pineda S, Heiman M, Kellis M, Steiner J, Nath A.Nat Commun. 2024 May 16;15(1):4163. doi: 10.1038/s41467-024-48488-7.
3. Structural basis for α-tubulin-specific and modification state-dependent glutamylation.
Mahalingan KK, Grotjahn DA, Li Y, Lander GC, Zehr EA, Roll-Mecak A.Nat Chem Biol. 2024 Apr 24. doi: 10.1038/s41589-024-01599-0.
4. Opposing actions of JIP4 and RILPL1 provide antagonistic motor force to dynamically regulate membrane reformation during lysosomal tubulation/sorting driven by LRRK2.
Bonet-Ponce L, Tegicho T, Beilina A, Kluss JH, Li Y, Cookson MR.bioRxiv [Preprint]. 2024 Apr 3:2024.04.02.587808. doi: 10.1101/2024.04.02.587808.
5. DELE1 promotes translation-associated homeostasis, growth, and survival in mitochondrial myopathy.
Lin HP, Petersen JD, Gilsrud AJ, Madruga A, D'Silva TM, Huang X, Shammas MK, Randolph NP, Li Y, Jones DR, Pacold ME, Narendra DP.bioRxiv. 2024 Feb 29:2024.02.29.582673. doi: 10.1101/2024.02.29.582673.
6. Regulation of NLGN3 and the Synaptic Rho-GEF Signaling Pathway by CDK5.
Jeong J, Han W, Hong E, Pandey S, Li Y, Lu W, Roche KW.J Neurosci. 2023 Nov 1;43(44):7264-7275. doi: 10.1523/JNEUROSCI.2309-22.2023. Epub 2023 Sep 12.
7. Some aspects of the life of SARS-CoV-2 ORF3a protein in mammalian cells.
Jiao S, Miranda P, Li Y, Maric D, Holmgren M.Heliyon. 2023 Aug 1;9(8):e18754. doi: 10.1016/j.heliyon.2023.e18754. eCollection 2023 Aug.
Terms: <2019 novel corona virus><2019 novel coronavirus><2019-nCoV><Acetylation><Anxiety><Autoregulation><CDK5><CDK5 gene><COVID-19 virus><COVID19 virus><Cell Division Kinase 5 Gene><CoV-2><CoV2><Core Facility><Devices><Disulfide Linkage><Generalized Growth><Goals><Growth><HPLC><Hares><High Performance Liquid Chromatography><High Pressure Liquid Chromatography><High Speed Liquid Chromatography><Homeostasis><Intramural Research><Investigators><LRRK2><LRRK2 gene><LRRK2 leucine-rich repeat kinase 2 gene><LRRK2 protein><Label><Lepus><Life><Mammalian Cell><Mass Photometry/Spectrum Analysis><Mass Spectrometry><Mass Spectroscopy><Mass Spectrum><Mass Spectrum Analyses><Mass Spectrum Analysis><Measurement><Membrane><Methods><Mitochondrial Myopathies><Modification><Molecular Weight><Motor><NIH><NINDS><National Institute of Neurological Diseases and Stroke><National Institute of Neurological Disorders and Stroke><National Institutes of Health><PARK8 protein><Paper><Parkinson disease 8 protein><Phosphorylation><Physiological Homeostasis><Post-Translational Modification Protein/Amino Acid Biochemistry><Post-Translational Modifications><Post-Translational Protein Modification><Post-Translational Protein Processing><Posttranslational Modifications><Posttranslational Protein Processing><Printing><Protein Modification><Protein Phosphorylation><Proteins><Proteomics><Regulation><Research Personnel><Research Support><Researchers><SARS corona virus 2><SARS-CO-V2><SARS-COVID-2><SARS-CoV-2><SARS-CoV2><SARS-associated corona virus 2><SARS-associated coronavirus 2><SARS-coronavirus-2><SARS-related corona virus 2><SARS-related coronavirus 2><SARSCoV2><Scientist><Services><Severe Acute Respiratory Coronavirus 2><Severe Acute Respiratory Distress Syndrome CoV 2><Severe Acute Respiratory Distress Syndrome Corona Virus 2><Severe Acute Respiratory Distress Syndrome Coronavirus 2><Severe Acute Respiratory Syndrome CoV 2><Severe Acute Respiratory Syndrome-associated coronavirus 2><Severe Acute Respiratory Syndrome-related coronavirus 2><Severe acute respiratory syndrome associated corona virus 2><Severe acute respiratory syndrome coronavirus 2><Severe acute respiratory syndrome related corona virus 2><Signal Pathway><Sorting><Stress><Synapses><Synaptic><System><TAR DNA-binding protein 43><TDP-43><TDP43><Techniques><Tissue Growth><Training><Translational Protein Modification><Translational Protein Processing><Translations><Ubiquitilation><Ubiquitination><Ubiquitinoylation><United States National Institutes of Health><Work><Wuhan coronavirus><alpha Tubulin><coronavirus disease 2019 virus><coronavirus disease-19 virus><dardarin><dardarin gene><dardarin protein><hCoV19><instrument><leucine-rich repeat kinase 2><mass spectrometer><membrane structure><nCoV2><nano><ontogeny><operation><operations><protein TDP-43><protein TDP43><synapse><translation><ubiquination><ubiquitin conjugation><α Tubulin>