Document text
Principal Investigator: Yuchao Chen
Organization: WELLSIM BIOMEDICAL TECHNOLOGIES, INC.
Fiscal Year: 2023
Award: $749,163
Funding agency: National Institute of General Medical Sciences
PROJECT SUMMARY / ABSTRACT
Extracellular vesicles (EVs) are small lipid bilayer particles secreted by most cell types, which encapsulates
biomolecules including lipids, proteins, and nucleic acids from cells of origin. Profiling of EV-derived biomarkers
provides a promising approach for diagnosis of various diseases. However, one of the primary challenges for
the research and application of EVs is a lack of a reliable method for efficient isolation of EVs and their
subpopulations from complex biofluids. Researchers in this field suffer from tedious workflow, long processing
time, high cost, and poor EV quality. To overcome the challenges, WellSIM proposes in this Phase II project,
which built upon the promising results generated from our Phase I project, to complete development of a Label-
free Extracellular-vesicle Automated Purification System (LEAP System) for isolation of total EVs or EV
subpopulations from a variety of biofluids in a non-invasive and cost-effective manner. Once developed, the
integrated LEAP System will offer excellent EV quality, throughput, and flexibility for EV isolation that can
outperform ultracentrifugation and other isolation techniques.
Terms: <Acceleration><Aliquot><Automation><Biologic Sciences><Biological Markers><Biological Sciences><Biomedical Technology><Bioscience><Blood Plasma><Blood Plasma Volume><Blood Serum><Cell Body><Cells><Clinic><Clinical><Complex><Computer software><Consumption><Culture Media><Development><Devices><Diagnosis><Disease><Disorder><Early Diagnosis><Encapsulated><Goals><Intervention><Intervention Strategies><Investigation><Investigators><Label><Laboratories><Life Sciences><Lipid Bilayers><Lipids><Manuals><Membrane><Methodology><Methods><Microfluidics><Molecular Sieve Chromatography><Nanoporous><Nucleic Acids><Outcome><Performance><Phase><Plasma><Plasma Serum><Plasma Volume><Population><Process><Proteins><Recovery><Research><Research Personnel><Researchers><Reticuloendothelial System, Serum, Plasma><Running><SBIR><Saliva><Sample Size><Sampling><Serum><Size Exclusion Chromatography><Small Business Innovation Research><Small Business Innovation Research Grant><Software><Source><Speed><System><Techniques><Technology><Testing><Time><Translations><Ultracentrifugation><Urine><bio-markers><biologic marker><biomarker><cell type><cost><cost effective><design><designing><developmental><early detection><extracellular vesicles><flexibility><flexible><growth media><improved><innovate><innovation><innovative><interest><interventional strategy><lipid bilayer membrane><membrane structure><nano particle><nano-sized particle><nanoparticle><nanosized particle><new technology><novel><novel technologies><particle><precision medicine><precision-based medicine><pressure><programs><success><tool><translation><µfluidic>